Protective effects of cyclopiazonic acid on ischemia-reperfusion injury in rabbit hearts

Journal of Cardiovascular Pharmacology
Martin AvellanalSantos Barrigon


The cardioprotective effects on myocardial ischemia of the sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) inhibitor, cyclopiazonic acid (CPA), were studied. We used the isolated arterially perfused interventricular septum of the rabbit heart submitted to 30-min global ischemia/30-min reperfusion. Mechanical [maximal increase in resting tension (MIRT), and the recovery of developed tension (RDT)], and biochemical parameters [creatine phosphokinase activity (CPK) in the effluent] were analyzed. CPA, 1 microM, perfused 30 min before the ischemia intervention significantly increased RDT by 54% and lessened MIRT by 66%. CPA also decreased CPK in the perfusate by 67.7 and 71.4% at 0-2 and 5-7 min of reperfusion, respectively. No additional benefits were shown either when the drug was perfused, both during ischemia and reperfusion, or with higher CPA concentrations (10-30 microM). The CPA cardioprotection was lost when the drug was present only during the reperfusion period. CPA exhibits functional and biochemical cardioprotective effects on myocardial ischemia. We postulated a decreased SR calcium contribution to the initial cytoplasmic calcium overload as the most probable mechanism involved.


Mar 28, 2003·Biochemical and Biophysical Research Communications·Briant E BurkeWolfgang H Dillmann
Apr 24, 2001·Pharmacology & Therapeutics·R ZucchiS Ronca-Testoni
Dec 25, 2002·Journal of Cardiovascular Pharmacology·Patricia RodriguezSantos Barrigon
Apr 24, 2009·Biochimica Et Biophysica Acta·Laure YatimeNatalya Fedosova
Jun 21, 2014·Journal of Molecular and Cellular Cardiology·Mariano N Di CarloAlicia Mattiazzi
Jun 26, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xiaoyin WangRakesh C Kukreja
Jan 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·M A Hassan TalukderJay L Zweier
Jul 17, 2007·American Journal of Physiology. Heart and Circulatory Physiology·M A Hassan TalukderJay L Zweier
Sep 18, 2012·Circulation Research·Chi Keung LamEvangelia G Kranias
Apr 27, 2018·Frontiers in Molecular Biosciences·Francesco Tadini-BuoninsegniMaria Rosa Moncelli


Feb 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G A Langer
Dec 1, 1989·Journal of Molecular and Cellular Cardiology·H Tanaka, K Shigenobu
Aug 1, 1973·Journal of Molecular and Cellular Cardiology·D J HearseE B Chain
Oct 1, 1994·Journal of Cardiovascular Pharmacology·E F du Toit, L H Opie
Feb 1, 1995·Journal of Molecular and Cellular Cardiology·D AtarE Marban
Sep 1, 1994·Journal of Molecular and Cellular Cardiology·A M LompréD Levitsky
Jan 26, 1994·Molecular and Cellular Biochemistry·M ChiesiS Grueninger
Aug 1, 1993·Circulation·R Zucchi
May 1, 1993·The Journal of Surgical Research·M B MitchellA H Harken
Jun 1, 1996·Journal of Molecular and Cellular Cardiology·Santos BarrigonG A Langer

Related Concepts

Myocardial Contraction
Calcium [EPC]
Ca(2+)-Transporting ATPase
Macro-Creatine Kinase
Adenosine Triphosphatases
Myocardial Ischemia
Coronary Arteriosclerosis

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.