PMID: 2508268Oct 2, 1989Paper

Protective effects of DL-alpha-lipoic acid on cadmium-induced deterioration of rat hepatocytes

Toxicology
L Müller

Abstract

The suitability of DL-alpha-lipoic acid (LA) to serve as an antidote in cadmium (Cd) toxicity in rat hepatocytes was investigated. Isolated hepatocytes were exposed to 200 and 450 microM Cd in the presence of 0.2, 1.0 and 5.0 mM LA, respectively. After 30 min of incubation various criteria of cell viability were monitored. Lipoic acid markedly diminished Cd uptake. Concomitantly, Cd-induced membrane injury, as reflected by the leakage of aspartate aminotransferase and sorbitol dehydrogenase (SDH) was decreased. Moreover, LA protected against intracellular toxic responses to Cd, such as a decrease in cellular SDH activity, a decrease in cellular acid soluble thiols, especially in total glutathione, a decrease in cellular urea and an increase in thiobarbituric acid (TBA) reactants, as a measure of lipid peroxidation. Most protective effects were seen in hepatocytes challenged with the lower Cd concentration and coincubated with 5 mM LA. In contrast, at 450 microM Cd even the highest LA concentration applied either did only reverse Cd-effects incompletely (SDH-response, TBA-reactants) or did not protect at all (Cd uptake, enzyme leakage, loss of glutathione). The data indicate that DL-alpha-lipoic acid serves as a protective tool ...Continue Reading

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Citations

Feb 1, 1994·Cell Biology and Toxicology·M Sugiyama
May 20, 1994·Toxicology·N M Elsayed
Sep 1, 1997·General Pharmacology·G P BiewengaA Bast
Jan 1, 1997·Free Radical Biology & Medicine·N HaramakiL Packer
Nov 13, 2004·The Journal of Nutritional Biochemistry·J Zempleni, D M Mock
Jan 1, 1991·Drug Metabolism Reviews·N Ballatori
Jan 1, 1990·Environmental Pollution·U ArndtB Ohnesorge
Sep 4, 2014·BioMed Research International·Bechan SharmaNikhat J Siddiqi
Sep 1, 1997·The Journal of Nutrition·J ZempleniD M Mock
Jan 2, 2021·International Journal of Environmental Research and Public Health·Chee Kong YapKhalid Awadh Al-Mutairi

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