Protective effects of epoxyeicosatrienoic acids on human endothelial cells from the pulmonary and coronary vasculature.

American Journal of Physiology. Heart and Circulatory Physiology
Anuradha DhanasekaranMeetha Medhora

Abstract

Epoxyeicosatrienoic acids (EETs) are cytochrome P-450 (CYP) metabolites synthesized from the essential fatty acid arachidonic acid to generate four regioisomers, 14,15-, 11,12-, 8,9-, and 5,6-EET. Cultured human coronary artery endothelial cells (HCAECs) contain endogenous EETs that are increased by stimulation with physiological agonists such as bradykinin. Because EETs are known to modulate a number of vascular functions, including angiogenesis, we tested each of the four regioisomers to characterize their effects on survival and apoptosis of HCAECs and cultured human lung microvascular endothelial cells (HLMVECs). A single application of physiologically relevant concentration of 14,15-, 11,12-, and 8,9-EET but not 5,6-EET (0.75-300 nM) promoted concentration-dependent increase in cell survival of HLMVECs and HCAECs after removal of serum. The lipids also protected the same cells from death via the intrinsic, as well as extrinsic, pathways of apoptosis. EETs did not increase intracellular calcium concentration ([Ca2+]i) or phosphorylate mitogen-activated protein kinase p44/42 when applied to these cells, and their protective action was attenuated by the phosphotidylinositol-3 kinase inhibitor wortmannin (10 microM) but not th...Continue Reading

References

Jun 19, 1987·European Journal of Pharmacology·M A CarrollJ C McGiff
May 1, 1995·The Journal of Clinical Investigation·D C ZeldinJ H Capdevila
May 1, 1996·Stroke; a Journal of Cerebral Circulation·N J AlkayedD R Harder
Jan 1, 1997·Advances in Experimental Medicine and Biology·J Z TanG H Gurtner
Aug 5, 1998·The American Journal of Physiology·A H StephensonA J Lonigro
Dec 29, 1998·The Journal of Biological Chemistry·B A RzigalinskiE F Ellis
May 5, 1999·Journal of Cardiovascular Pharmacology·J V MombouliW F Graier
Oct 16, 1999·Nature·B FisslthalerR Busse
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D ZhuE R Jacobs
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D H Munzenmaier, D R Harder
Jul 19, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M M Medhora
Aug 26, 2000·Archives of Toxicology·C D AnuradhaS Hirano
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·E R Jacobs, D C Zeldin
Aug 18, 2001·Molecular and Cellular Biology·J K ChenR C Harris
Feb 14, 2002·Trends in Biochemical Sciences·Timothy A McKinseyEric N Olson
Jun 8, 2002·Stroke; a Journal of Cerebral Circulation·Nabil J AlkayedPatricia D Hurn
Jun 19, 2002·Journal of Applied Physiology·Daling ZhuElizabeth R Jacobs
Sep 25, 2002·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Andreas ZakrzewiczAxel R Pries
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Meetha MedhoraDavid R Harder
Feb 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·U Ruth MichaelisRudi Busse
Jul 5, 2003·Science·Alireza AlaviDavid A Cheresh
Dec 8, 2004·Archives of Biochemistry and Biophysics·Martin Spiecker, James K Liao

❮ Previous
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Citations

Jan 16, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tracy C DelozierJoyce A Goldstein
Apr 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Garrett J GrossKasem Nithipatikom
Feb 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Shilin YangDarryl C Zeldin
Dec 7, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Anuradha DhanasekaranMeetha Medhora
Feb 26, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Meetha MedhoraElizabeth R Jacobs
Feb 3, 2012·Physiological Reviews·John D Imig
Jun 23, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Nandita C GuptaNabil J Alkayed
Oct 2, 2012·Annual Review of Pharmacology and Toxicology·Christophe Morisseau, Bruce D Hammock
Jul 16, 2013·Translational Stroke Research·Catherine M DavisNabil J Alkayed
Nov 7, 2015·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·David C EllinsworthDavid D Gutterman
Nov 7, 2009·Journal of Molecular and Cellular Cardiology·Yangmei DengCraig R Lee
Apr 2, 2009·Journal of Molecular and Cellular Cardiology·Sreedhar BodigaMeetha M Medhora
May 14, 2009·The American Journal of Pathology·Alexis N SimpkinsJohn D Imig
Nov 6, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·V Lamounier-ZepterI Morano
Dec 3, 2014·Prostaglandins & Other Lipid Mediators·Susan ZelaskoAditi Das
Jun 11, 2016·Drug Metabolism Reviews·Michael Murray
Mar 5, 2010·Journal of Cardiovascular Pharmacology and Therapeutics·Kasem Nithipatikom, Garrett J Gross
Jun 4, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Jian CaoNader G Abraham
Sep 30, 2016·Pulmonary Circulation·Sachindra Raj JoshiSachin A Gupte
May 20, 2018·The Journal of Biological Chemistry·Sang-Kyu ParkWilliam B Campbell
Feb 4, 2016·Circulation Research·David D GuttermanAndreas M Beyer
Dec 20, 2007·Journal of Cardiovascular Pharmacology·Kathryn M GauthierWilliam B Campbell
Jan 13, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Anuradha DhanasekaranElizabeth R Jacobs
Jun 9, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sherif M Shoieb, Ayman O S El-Kadi
Dec 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hanfeng GuanJun Xiao
Nov 26, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ahmed A Elmarakby
Feb 7, 2015·British Journal of Clinical Pharmacology·L YangI B Wilkinson
Dec 16, 2019·Prostaglandins & Other Lipid Mediators·Malvina Hoxha, Bruno Zappacosta
Aug 16, 2017·Research in Veterinary Science·Vengai Mavangira, Lorraine M Sordillo

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