Protective effects of grape seed extract against oxidative and nitrative damage of plasma proteins

International Journal of Biological Macromolecules
M BijakP Nowak

Abstract

Oxidative stress, vascular inflammation, endothelial dysfunction plays a crucial role in the pathogenesis of cardiovascular diseases. The aim of our in vitro study was to examine the antioxidative properties of grape seed extract, and its potential protective effect on the haemostatic function of human fibrinogen under oxidative stress conditions, induced by peroxynitrite (100 μM). The preincubation of plasma with the tested extract (0.5-50 μg/ml or 0.5-300 μg/ml) reduced the formation of 3-nitrotyrosine and diminished oxidation of thiol groups in plasma proteins. The low concentrations (0.5-50 μg/ml) of grape seed extract also decreased the level of carbonyl groups, however at higher concentrations (100-300 μg/ml) this effect was not observed. Furthermore, grape seed extract counteracted the inhibitory effect of peroxynitrite on human plasma clotting. The results obtained in this study indicate that components of the grape seed extract posses antioxidative properties and may be promising substances for the creation of new dietary supplements.

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Citations

Jan 30, 2013·International Journal of Biological Macromolecules·Michał BijakPaweł Nowak
Jul 26, 2015·International Journal of Biological Macromolecules·Joanna SalukHalina M Zbikowska
Apr 4, 2015·Oxidative Medicine and Cellular Longevity·Natalia CichońJoanna Saluk
Sep 18, 2012·International Journal of Biological Macromolecules·Paola PersicoPierfrancesco Cerruti
Jun 9, 2016·International Journal of Biological Macromolecules·Michał Błażej Ponczek
Aug 1, 2016·Chemico-biological Interactions·Michal BijakPawel Nowak
Feb 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michal BijakJoanna Saluk-Bijak
Jan 10, 2019·Nutrients·Michal BijakJacek Golanski
Jun 22, 2018·Drug and Chemical Toxicology·Mustafa EvcimenMehmet Sukru Gulay
Aug 12, 2017·BMC Complementary and Alternative Medicine·Michal Bijak, Joanna Saluk-Bijak
Dec 8, 2020·Frontiers in Physiology·Maria J XavierLuisa M P Valente
Aug 15, 2013·Bioorganic Chemistry·Joanna SalukBarbara Wachowicz
Aug 27, 2017·International Journal of Biological Macromolecules·Michal BijakJoanna Saluk-Bijak

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