PMID: 22587922May 17, 2012Paper

Protective effects of heparin against increased permeability of endothelial cells induced by lipopolysaccharide

Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
Yi-ran MaoXiao-chun Ma

Abstract

To evaluate the effects of heparin on the changes in permeability and cytoskeleton of cultured human umbilical vascular endothelial cells (HUVECs) induced by lipopolysaccharide (LPS). HUVECs were cultured in vitro and randomly assigned to blank control group, LPS group, heparin group, and LPS + heparin group, n=8. Cell viability was determined by methyl thiazolyl tetrazolium (MTT) method. Endothelial permeability was measured with the Transwell chamber models. F-actin of cytoskeletons was assayed with fluorescein isothiocyanate (FITC)-phalloidine. Compared with the blank control group, 10 mg/L and 100 mg/L of LPS significantly inhibited cell viability (0.695±0.015, 0.476±0.030 vs. 0.860±0.053, P<0.05 and P<0.01). Heparin 100 kU/L also inhibited cell viability (0.675±0.030 vs. 0.840±0.023, P<0.05). Increase in permeability of endothelium was induced by 10 mg/L of LPS, and it peaked at 4 hours, there was significant difference compared with blank control group (5.882±0.101 vs. 4.489±0.015, P<0.05). At the same time, LPS led to the reorganization of F-actin cytoskeleton and the formation of stress fibers. LPS+heparin decreased the increase in permeability of endothelium at 2-12 hours (2 hours: 4.382±0.053 vs. 5.084±0.129, 4 hours:...Continue Reading

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