Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease.

Stem Cell Research & Therapy
Deliang ZhuJiansu Chen

Abstract

Retinitis pigmentosa (RP) is an inherited retinal disease characterized by progressive loss of photoreceptor cells. This study aim at exploring the effect of retinal pigment epithelium (RPE) derived from human-induced pluripotent stem cell (hiPSC-RPE) on the retina of retinal degeneration 10 (rd10) mice, which are characterized with progressive photoreceptor death. We generated RPE from hiPSCs by sequential supplementation with retinal-inducing factors and RPE specification signaling factors. The three-dimensional (3D) spheroid culture method was used to obtain optimal injectable hiPSC-RPE cells. Subretinal space transplantation was conducted to deliver hiPSC-RPE cells into the retina of rd10 mice. Neurotrophic factor secretion from transplanted hiPSC-RPE cells was detected by enzyme-linked immunosorbent assay (ELISA). Immunostaining, Western blotting, electroretinography (ERG), and visual behavior testing were performed to determine the effects of hiPSC-RPE on the retinal visual function in rd10 mice. Our data demonstrated that hiPSC-RPE cells exhibited classic RPE properties and phenotype after the sequential RPE induction from hiPSCs. hiPSC-RPE cells co-cultured with mouse retinal explants or retinal ganglion cells 5 (RGC5) ...Continue Reading

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Citations

Jan 28, 2021·International Journal of Molecular Sciences·Jongmin KimDong Woo Cho
Aug 6, 2021·Biologics : Targets & Therapy·Amit Sharma, Bithiah Grace Jaganathan

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Methods Mentioned

BETA
fluorescence microscopy
flow cytometry
enzyme-linked immunosorbent assay
ELISA
electrophoresis

Software Mentioned

ERG analyzer
FACSDiva
ANY
SPSS
GraphPad PRISM
Maze

Related Concepts

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