Protective effects of lycopene against H2O2-induced oxidative injury and apoptosis in human endothelial cells

Cardiovascular Drugs and Therapy
XiangYu TangJunHua Lin

Abstract

Oxidative stress is considered to be a major factor contributing to damage of endothelial cells, and is an important component of the etiology of atherosclerosis. In this study, we investigated the effects of lycopene on the oxidative injury and apoptosis of endothelial cells induced by H(2)O(2), and the effects of lycopene on the expression of p53, caspase-3 mRNA in injured cells. In the H(2)O(2) group, endothelial cells were incubated with 400 microM H(2)O(2). In lycopene groups, endothelial cells were pretreated with different concentrations of lycopene then exposed to 400 microM H(2)O(2). In the drug control group, cells were pretreated with probucol then incubated with H(2)O(2). The effects of different concentrations of lycopen on the extent of oxidative injury to the cells were evaluated. The growth conditions and morphological changes of the cells were observed with an inverted microscope. The level of oxidative injury to cells was determined by measuring malondialdehyde (MDA) levels; the viability of cells was detected by MTT assays; the nuclear morphology of cells was observed by Hoechst staining; the apoptotic ratio of cells was measured by flow cytometry; and the expressions of p53 and caspase-3 mRNA were investigat...Continue Reading

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