Protective effects of reactive functional groups on chondrocytes in photocrosslinkable hydrogel systems

Acta Biomaterialia
M BartnikowskiTravis J Klein

Abstract

Photocrosslinkable hydrogels are frequently used in cartilage tissue engineering, with crosslinking systems relying on cytotoxic photoinitiators and ultraviolet (UV) light to form permanent hydrogels. These systems are rarely assessed in terms of optimization of photoinitiator or UV dosage, with non-cytotoxic concentrations from literature deemed sufficient. We hypothesized that the number of reactive functional groups present within a hydrogel polymer is highly relevant when crosslinking, affording cytoprotection to chondrocytes by preferentially interacting with the highly reactive radicals that are formed during UV-mediated activation of a photoinitiator. This was tested using two photocrosslinkable hydrogel systems: gelatin methacrylamide (GelMA) and gellan gum methacrylate (GGMA). We further assessed the effects of two different UV dosages on chondrocyte differentiation while subject to a single photoinitiator dosage in the GGMA system. Most notably, we found that a higher ratio of reactive groups to photoinitiator molecules offers cytoprotective effects, and future developments in photocrosslinkable hydrogel technology may involve assessment of such ratios. In contrast, we found there to be no effect of UV on chondrocyte ...Continue Reading

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Citations

Jul 19, 2016·Biomaterials Science·L R StevensM In Het Panhuis
Jan 20, 2018·Regenerative Medicine·Ji XiongfaXiao Jun
Aug 20, 2019·Advanced Materials·Paulina Nuñez BernalRiccardo Levato
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Stephen PahoffDietmar W Hutmacher
Dec 6, 2018·Nature Reviews. Rheumatology·Magali Cucchiarini, Henning Madry
Mar 4, 2020·Carbohydrate Polymers·Henna CassimjeeViness Pillay
Mar 7, 2021·Acta Biomaterialia·Qiang ZhangZili Ge
Oct 10, 2016·ACS Biomaterials Science & Engineering·Khoon S LimTim B F Woodfield

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