Protective effects of Sm-p80 in the presence of resiquimod as an adjuvant against challenge infection with Schistosoma mansoni in mice.

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Gul AhmadAfzal A Siddiqui

Abstract

To determine the prophylactic efficacy of an Sm-p80-based vaccine formulation against challenge infection with Schistosoma mansoni in mice using an approach comprising of initial priming with DNA and boosting with recombinant protein in the presence of resiquimod (R848) as an adjuvant. In the first experiment (prime-boost approach), mice were primed with Sm-p80-pcDNA3 (week 0) and boosted at weeks 4 and 8 with recombinant Sm-p80 formulated in resiquimod (R848). Each mouse in the control group first received only pcDNA3 and was boosted with R848. In the second set of experiments (recombinant protein approach), mice were immunized (week 0) and boosted (weeks 4 and 8) with rSm-p80 formulated in R848. Animals of the control group in this series of experiments received only R848 at 0, 4, and 8 weeks. All of the animals from both the 'prime-boost' and 'recombinant protein' groups were challenged with cercariae of S. mansoni, 4 weeks after the last immunization. The mice were sacrificed 6 weeks post-challenge and the reductions in worm burden and egg production were determined. Sm-p80-specific antibody titers were estimated in the mice sera by ELISA. Cytokine mRNA and protein production by proliferating splenocytes in response to in v...Continue Reading

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Citations

Apr 18, 2013·PLoS Neglected Tropical Diseases·Xuefeng WangChuan Su
Jan 1, 2014·Vaccines·Rachel StephensonIstvan Toth
Dec 15, 2015·Expert Review of Vaccines·Adebayo J MolehinAfzal A Siddiqui
Mar 31, 2015·Experimental Parasitology·Qyana GriffithLisa Ganley-Leal
Nov 4, 2011·Human Vaccines·Afzal A SiddiquiLisa Ganley-Leal
Jan 1, 2014·Human Vaccines & Immunotherapeutics·Souvik KarmakarAfzal A Siddiqui
Mar 26, 2015·Frontiers in Immunology·Cristina Toscano FonsecaClarice Carvalho Alves
Oct 8, 2016·Parasites & Vectors·Biniam Mathewos TebejeDonald P McManus
Dec 15, 2017·Annals of the New York Academy of Sciences·Workineh TorbenAfzal A Siddiqui
Jul 13, 2018·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Weidong ZhangAfzal A Siddiqui
Jul 28, 2013·Expert Review of Vaccines·John P Vasilakos, Mark A Tomai
Jan 23, 2018·Expert Review of Vaccines·Gebeyaw Getnet MekonnenJavier Sotillo
Aug 7, 2021·Vaccines·Ndibonani Kebonang QokoyiAbidemi Paul Kappo

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