Protective effects of tiotropium alone or combined with budesonide against cadmium inhalation induced acute neutrophilic pulmonary inflammation in rats

PloS One
Shiwei ZhaoWenhui Zhang

Abstract

As a potent bronchodilator, the anti-inflammatory effects of tiotropium and its interaction with budesonide against cadmium-induced acute pulmonary inflammation were investigated. Compared to values obtained in rats exposed to cadmium, cytological analysis indicated a significant decrease of total cell and neutrophil counts and protein concentration in bronchoalveolar lavage fluid (BALF) in rats pretreated with tiotropium (70μg/15ml or 350μg/15ml). Zymographic tests showed a decrease of MMP-2 activity in BALF in rats pretreated only with high concentration of tiotropium. Histological examination revealed a significant decrease of the severity and extent of inflammatory lung injuries in rats pretreated with both tested concentrations of tiotropium. Though tiotropium (70μg/15ml) or budesonide (250μg/15ml) could not reduce cadmium-induced bronchial hyper-responsiveness, their combination significantly decreased bronchial contractile response to methacholine. These two drugs separately decreased the neutrophil number and protein concentration in BALF but no significant interaction was observed when both drugs were combined. Although no inhibitory effects on MMP-2 and MMP-9 was observed in rats pretreated with budesonide alone, the ...Continue Reading

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Citations

Dec 4, 2019·Immunopharmacology and Immunotoxicology·Nikoo Hossein-KhannazerMohammad Noorisepehr
Feb 20, 2021·International Journal of Chronic Obstructive Pulmonary Disease·Luigino CalzettaPaola Rogliani
Nov 3, 2021·Tuberculosis and Respiratory Diseases·Tai Joon AnHyoung Kyu Yoon

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Methods Mentioned

BETA
protein
Protein Assay
ELISA
bronchoalveolar lavage
bronchoalveolar
lavage
zymography

Software Mentioned

Chart
Image J Image Processing and Analysis
GraphPad Prism

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