Protective effects of vascular endothelial growth factor in cultured brain endothelial cells against hypoglycemia

Metabolic Brain Disease
Fei ZhaoYuwu Zhao

Abstract

Hypoglycemia is a common and serious problem among patients with type 1 diabetes receiving treatment with insulin. Clinical studies have demonstrated that hypoglycemic edema is involved in the initiation of hypoglycemic brain damage. However, the mechanisms of this edema are poorly understood. Vascular endothelial growth factor (VEGF), a potent regulator of blood vessel function, has been observed an important candidate hormone induced by hypoglycemia to protect neurons by restoring plasma glucose. Whether VEGF has a protective effect against hypoglycemia-induced damage in brain endothelial cells is still unknown. To investigate the effects of hypoglycemia on cerebral microvascular endothelial cells and assess the protective effect of exogenous VEGF on endothelial cells during hypoglycemia, confluent monolayers of the brain endothelial cell line bEnd.3 were treated with normal (5.5 mM glucose), hypoglycemic (0, 0.5, 1 mM glucose) medium or hypoglycemic medium in the presence of VEGF. The results clearly showed that hypoglycemia significantly downregulated the expression of claudin-5 in bEnd.3 cells, without affecting ZO-1 and occludin expression and distribution. Besides, transendothelial permeability significantly increased un...Continue Reading

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Citations

Apr 19, 2016·Brain Research·Nurul Adhwa RahmanSiti Hanna Muharram
Jan 7, 2017·Journal of Neurosurgery·Martin PiazzaPrashant Chittiboina
Jul 2, 2019·Fluids and Barriers of the CNS·Shujun GeJoel S Pachter
Dec 12, 2019·Frontiers in Pharmacology·Simona Federica SpampinatoMariagrazia Grilli
Feb 1, 2019·International Journal of Molecular Sciences·Shotaro Michinaga, Yutaka Koyama
Aug 16, 2019·Cellular and Molecular Neurobiology·Takashi FujimotoTakayuki Matsuo

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Methods Mentioned

BETA
immunoprecipitation
protein assay
electrophoresis

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