Protective efficacy evaluation induced by recombinant protein LHD-Sj23-GST of Schistosoma japonicum emulsified with three different adjuvants

Parasite Immunology
Z ZhuJ Lin

Abstract

As a complement to chemotherapy and other control approaches, the development of an effective vaccine is necessary to combat Schistosomiasis japonica that remains a serious public health problem in China. In the present study, mice were vaccinated with purified recombinant protein LHD-Sj23-GST (large hydrophilic domain of 23 kDa antigen of S. japonicum fused with Sj26GST) emulsified with Freund's adjuvant (FA), Montanide ISA 206 and Montanide ISA 70 M and challenged with cercariae, the protective efficacy induced by the recombinant protein was evaluated, and the LHD-Sj23-GST-specific IgG and its subtypes were determined. The result revealed that a significant worm burden reduction (58.8%, 26.3% and 54.3%; P<0.05) was obtained in mice vaccinated with LHD-Sj23-GST emulsified with three different adjuvants compared to those mice treated with respective adjuvant only. ELISA test suggested that the high-level production of LHD-Sj23-GST-specific IgG1, IgG2a and IgG3 antibodies may participate in protecting against schistosome infection.

References

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Citations

Jan 30, 2013·Experimental Parasitology·A M Martínez-IbeasM Y Manga-González
Nov 2, 2014·Parasitology·Hong You, Donald P McManus
Oct 8, 2016·Parasites & Vectors·Biniam Mathewos TebejeDonald P McManus
Jan 23, 2018·Expert Review of Vaccines·Gebeyaw Getnet MekonnenJavier Sotillo
Dec 3, 2016·PLoS Neglected Tropical Diseases·Daiane P OldigesCarlos E Suarez

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