PMID: 9420203Jan 7, 1998Paper

Protective efficacy of DNA vaccines against duck hepatitis B virus infection

Journal of Virology
M TriyatniChristopher J Burrell

Abstract

The efficacy of DNA vaccines encoding the duck hepatitis B virus (DHBV) pre-S/S and S proteins were tested in Pekin ducks. Plasmid pcDNA I/Amp DNA containing the DHBV pre-S/S or S genes was injected intramuscularly three times, at 3-week intervals. All pre-S/S and S-vaccinated ducks developed total anti-DHBs and specific anti-S antibodies with similar titers reaching 1/10,000 to 1/50,000 and 1/2,500 to 1/4,000, respectively, after the third vaccination. However, following virus challenge, significant differences in the rate of virus removal from the bloodstream and the presence of virus replication in the liver were found between the groups. In three of four S-vaccinated ducks, 90% of the inoculum was removed between <5 and 15 min postchallenge (p.c.) and no virus replication was detected in the liver at 4 days p.c. In contrast, in all four pre-S/S-vaccinated ducks, 90% of the inoculum was removed between 60 and 90 min p.c. and DHBsAg was detected in 10 to 40% of hepatocytes. Anti-S serum abolished virus infectivity when preincubated with DHBV before inoculation into 1-day-old ducklings and primary duck hepatocyte cultures, while anti-pre-S/S serum showed very limited capacity to neutralize virus infectivity in these two system...Continue Reading

References

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Citations

Jul 23, 2004·Expert Opinion on Biological Therapy·Jesus PrietoM Gabriela Kramer
Dec 22, 2006·Expert Review of Vaccines·Stanislas Pol, Marie-Louise Michel
Sep 5, 2002·Reviews in Medical Virology·Susanne BeckebaumGuido Gerken
Mar 11, 2005·Expert Review of Vaccines·Liz HaygreenPete Kaiser
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Wendy K FosterAllison R Jilbert
Jan 17, 2007·Expert Opinion on Biological Therapy·Gloria Gonzalez-AseguinolazaAstrid Paneda
Aug 27, 2005·World Journal of Gastroenterology : WJG·Jian-Er LongDi Qu
May 4, 2012·Expert Review of Vaccines·Veronica L Fowler, Paul V Barnett
Aug 9, 2006·Clinical and Vaccine Immunology : CVI·Sheryl L GaresKlaus S Gutfreund
May 30, 2019·Frontiers in Immunology·Martha WiumAnnelise Botes
Sep 6, 2000·Veterinary Immunology and Immunopathology·L A Babiuk van Drunnen Littel-van den Hur

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