Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice.

Infection and Immunity
Deyan LuoXiliang Wang

Abstract

This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccine into BALB/c mice elicited markedly both humoral and cellular immune responses. The specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the dual-gene DNA vaccine elicited a strong T-cell proliferative response and induced a large amount of gamma interferon-producing T cells upon restimulation in vitro with recombinant fusion protein L7/L12-Omp16, suggesting the induction of a typical T-helper-1-dominated immune response in vivo. This divalent DNA vaccine could also induce a significant level of protection against challenge with the virulent strain B. abortus 544 in BALB/c mice. Furthermore, the protection level induced by the divalent DNA vaccine was significantly higher than that induced by the univalent DNA vaccines pcDNA3.1-L7/L12 or pcDNA3.1-Omp16. Taken together, the results of this study verify for the first time that the Omp16 gene can be a candidate target for a ...Continue Reading

References

Jan 1, 1993·Infection and Immunity·X Jiang, C L Baldwin
Jan 1, 1997·Annual Review of Immunology·J J DonnellyM A Liu
Aug 24, 1999·Infection and Immunity·A TiborJ J Letesson
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Reinhold SchirmbeckJörg Reimann
Apr 22, 2004·Veterinary Research·Ignacio MoriyónJosé María Blasco
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steven R LittleRobert Langer
Aug 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Margaret LiuPatrick Squiban
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guillermo H GiambartolomeiMario T Philipp
Feb 5, 2005·Cancer Immunology, Immunotherapy : CII·Dean Johnston, Jean-Claude Bystryn
Aug 17, 2005·International Immunopharmacology·Ema PaulovicováDanica Mislovicová

❮ Previous
Next ❯

Citations

Dec 12, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J XuZ Chen
Oct 16, 2010·Nature Reviews. Microbiology·Jennifer BarrilaCheryl A Nickerson
Feb 9, 2011·The Journal of Infectious Diseases·Duangchan SuwannasaenGanjana Lertmemongkolchai
Nov 5, 2010·Clinical and Vaccine Immunology : CVI·Prathiba KurupatiChit Laa Poh
Feb 12, 2009·Pakistan Journal of Biological Sciences : PJBS·H AbtahiG Mosayebi
Oct 20, 2009·Vaccine·Shan Lu, Shixia Wang
Jul 19, 2013·BioMed Research International·Eric Daniel Avila-CalderónAraceli Contreras-Rodríguez
Mar 15, 2016·Immunotherapy·Forough Golsaz-Shirazi, Fazel Shokri
Jul 8, 2015·Applied Microbiology and Biotechnology·Prachiti AfleySubodh Kumar
Feb 26, 2010·FEMS Microbiology Reviews·Stuart D PerkinsHelen S Atkins
Jul 15, 2015·Veterinary Research·Elaine M S DornelesAndrey P Lage
Jan 5, 2010·The Journal of Veterinary Medical Science·Tai YangYu-Ling Li
Nov 21, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Tooba Abbassi-DaloiiMojtaba Tahmoorespur
Sep 17, 2011·Expert Review of Vaccines·Sérgio Costa OliveiraJuliana Cassataro
Aug 24, 2007·Clinical and Vaccine Immunology : CVI·M Victoria DelpinoPablo C Baldi
Nov 16, 2016·PloS One·Tatiane F CarvalhoRenato L Santos
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gilson Costa MacedoSergio Costa Oliveira
Oct 31, 2017·BioMed Research International·Leonardo GómezAngel Oñate

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.