Protective immunity induced by rPb27 of Paracoccidioides brasiliensis

Vaccine
B S ReisA M Goes

Abstract

A cDNA coding for an antigenic protein (rPb27) from the pathogenic fungus Paracoccidioides brasiliensis was cloned and its protective activity was determined against paracoccidioidomycosis (PCM). The cDNA sequence contained an open reading frame (ORF) of 660 base pairs encoding a protein of 219 amino acids with a predicted molecular weight of 25kDa. The deduced amino acid sequence exhibited 100% identity to the 27kDa P. brasiliensis hypothetic protein (access number AA49615). The complete coding cDNA was cloned into a pGEX 4T-2 plasmid and expressed in Escherichia coli as a glutathione-S-transferase-tagged (GST) recombinant protein. Mice immunized with purified rPb27 were able to develop high levels of IgG2b, moderate levels of IgG1 and low levels of IgG2a. At the same time the levels of TGF-beta and IFN-gamma were high while a very low production of IL-10 was verified. Using confocal microscopy with anti-rPb27 mouse serum against P. brasiliensis yeast forms, surface and cytosolic staining pattern were observed. Moreover, immunization of mice with this antigen induced a significant degree of protection in the lungs (93%), liver (93%) and spleen (100%) at 60 days after challenge with infection. Thus, the granulomatous lesions re...Continue Reading

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Citations

Nov 26, 2015·Microbes and Infection·Elis Araujo MoraisAlfredo Miranda de Goes
Sep 29, 2011·Fungal Genetics and Biology : FG & B·S García BlancoA M García
Dec 21, 2010·Revista iberoamericana de micología·Gioconda San-Blas, Eva Burger
Oct 24, 2015·Diagnostic Microbiology and Infectious Disease·Julhiany de Fátima da SilvaMaria José Soares Mendes-Giannini
Dec 5, 2015·Frontiers in Microbiology·Haroldo C de OliveiraMaria J S Mendes-Giannini
Dec 5, 2014·PLoS Neglected Tropical Diseases·Ana Claudia Paiva Alegre-MallerMaria Cristina Roque-Barreira
Mar 6, 2012·PLoS Neglected Tropical Diseases·Glauce M G RittnerLuiz R Travassos
Jul 16, 2016·Future Microbiology·Cyntia Silva FerreiraBreno de Mello Silva
Jul 20, 2017·MBio·Fabrício F FernandesMaria Cristina Roque-Barreira
Sep 7, 2016·PloS One·Fabrício Freitas FernandesAdemilson Panunto-Castelo
Apr 8, 2009·Mycopathologia·Estefânia Mara do Nascimento MartinsAlfredo Miranda Goes

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