Protective lactogenic immunity conferred by an edible peptide vaccine to bovine rotavirus produced in transgenic plants

The Journal of General Virology
A WigdorovitzM V Borca

Abstract

Vaccines produced in transgenic plants constitute a promising alternative to conventional immunogens, presenting the possibility of stimulating secretory and systemic immunity against enteric pathogens when administered orally. Protection against enteric pathogens affecting newborn animals requires, in most cases, the stimulation of lactogenic immunity. Here, the group presents the development of an experimental immunogen based on expression of an immunorelevant peptide, eBRV4, of the VP4 protein of bovine rotavirus (BRV), which has been described as harbouring at least one neutralizing epitope as well as being responsible for the adsorption of the virus to epithelial cells. The eBRV4 epitope was efficiently expressed in transgenic alfalfa as a translational fusion protein with the highly stable reporter enzyme beta-glucuronidase (betaGUS), which served as a carrier, stabilized the synthesized peptide and facilitated screening for the higher expression levels in plants. Correlation of expression of the eBRV4 epitope in plants with those presenting the highest betaGUS activities was confirmed by a Western blot assay specific for the BRV peptide. The eBRV4 epitope expressed in plants was effective in inducing an anti-rotavirus an...Continue Reading

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Citations

Nov 30, 2005·Transgenic Research·Eric SolerLouis-Marie Houdebine
Jul 16, 2008·Veterinary Research Communications·K DhamaS V S Malik
Apr 6, 2006·Animal Health Research Reviews·J RiceP Shewen
Apr 2, 2005·Viral Immunology·Judith M BallRebecca D Parr
May 10, 2005·Immunology and Cell Biology·María José Dus Santos, Andrés Wigdorovitz
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Nov 22, 2005·Expert Opinion on Drug Delivery·Stephen J Streatfield
Apr 10, 2009·Biotechnology Advances·Siddharth TiwariRakesh Tuli
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Feb 21, 2006·Journal of Virological Methods·Liang-Kai HuangHung-Jen Liu
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May 31, 2017·Vaccines·Christopher ConchaCristian Ibáñez
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