Protective Mechanism of Sulforaphane on Cadmium-Induced Sertoli Cell Injury in Mice Testis via Nrf2/ARE Signaling Pathway.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Shu-Hua YangJian-Bin He

Abstract

The present study evaluated the mechanism underlying the protective effect of sulforaphane (SFN) on cadmium (Cd)-induced Sertoli cell (TM4 cells) injury in mice. The apoptosis rate of cells in each group was detected by flow cytometry. It was determined the effect of SFN on the expression of downstream molecular targets of Nrf2/ARE axis and on the lipid peroxide content. The related genes involved in the nuclear factor E2-related factor 2(Nrf2)/antioxidant response element (ARE) signaling pathway were evaluated by RT-PCR; for example, the mRNA expression levels of Nrf2, heme oxygenase-1 (HO-1), glutathione peroxidase (GSH-Px), quinone oxidoreductase 1 (NQO1), and γ-glutamylcysteine synthetase (γ-GCS), while the protein expression levels were assessed by Western blot. Our results showed that the mRNA and protein expression levels of Nrf2, HO-1, NQO1, GSH-Px, and γ-GCS were increased in various degree when the Sertoli cells were to added different concentrations of SFN. Our results also showed that SFN reduced the apoptosis rate, increased the activity of T-SOD, inhibited the increase of the MDA content caused by Cd. Meanwhile, SFN could increase the mRNA and protein expression levels of Nrf2, HO-1 and NQO1 and reduced the mRNA a...Continue Reading

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Citations

Sep 29, 2018·Basic & Clinical Pharmacology & Toxicology·Jerrold J Heindel
Sep 4, 2019·Annals of Clinical and Translational Neurology·Fayin LiPengfei Gao
Dec 2, 2019·Biological Trace Element Research·Milad AshrafizadehSaeed Samarghandian
Feb 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Susana M Cardoso
Feb 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Annie Langston-CoxSarah A Marshall
Dec 14, 2018·International Journal of Molecular Sciences·Rafa S AlmeerAhmed E Abdel Moneim
Jun 4, 2020·Environmental Science and Pollution Research International·Shirin MoradkhaniAmir Nili-Ahmadabadi
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Dec 23, 2021·Biomédica : revista del Instituto Nacional de Salud·Omid JazayeriFereshteh Mir Moammadrezaei

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Methods Mentioned

BETA
flow cytometry
MDA
electrophoresis
PCR

Software Mentioned

primer
SPSS17

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis