Protective mechanisms of pycnogenol in ethanol-insulted cerebellar granule cells

Journal of Neurobiology
Kendra I Siler-MarsiglioM B Heaton

Abstract

Pycnogenol (PYC), a patented combination of bioflavonoids extracted from the bark of French maritime pine (Pinus maritima), inhibits apoptosis and necrosis of developing neurons exposed acutely to ethanol (EtOH). The present study shows that the protective mechanisms of PYC in EtOH-exposed postnatal day 9 cerebellar granule cells (P9 CGCs) include (1) reduction of reactive oxygen species (ROS) production; (2) counteraction of suppressed copper/zinc superoxide dismutase (Cu/Zn SOD) and glutathione peroxidase/reductase (GSH-Px/GSSG-R) system activities; (3) upregulation of Cu/Zn SOD protein expression; (4) mitigation of the EtOH-mediated exacerbation of catalase (CAT) activity; and, (5) specific binding and inhibition of active caspase-3. These results indicate that the mechanisms by which PYC antagonizes EtOH-induced oxidative stress include oxidant scavenging and modulation of endogenous, cellular proteins. Using findings from the present and previous studies, a model delineating the mechanisms of EtOH effects on the system of antioxidant enzymes in developing CGCs is presented.

References

Oct 1, 1992·Alcoholism, Clinical and Experimental Research·K GillR A Deitrich
Jul 7, 1992·Biochemical Pharmacology·C M AragonZ Amit
Dec 1, 1991·Pharmacology & Therapeutics·L D DeLeve, N Kaplowitz
Aug 1, 1989·Alcoholism, Clinical and Experimental Research·R J Sokol, S K Clarren
Oct 1, 1993·Alcoholism, Clinical and Experimental Research·N J PantazisJ R West
May 20, 1998·Pharmacology, Biochemistry, and Behavior·F T CrewsA Day
Jan 28, 1999·Methods in Molecular Biology·C E PippengerD Armstrong
Jun 17, 1999·Frontiers in Bioscience : a Journal and Virtual Library·G I HendersonS Schenker
Oct 6, 1999·European Journal of Clinical Nutrition·M KielyP A Morrissey
Dec 6, 2000·Indian Journal of Pediatrics·G GathwalaK Sangwan
Jan 5, 2002·Mental Retardation and Developmental Disabilities Research Reviews·J W OlneyC Ikonomidou
Feb 13, 2002·Alcohol·J A Mennella, P L Garcia-Gomez
Mar 16, 2002·Neurobiology of Disease·John W OlneyKevin A Roth
Jul 16, 2002·Brain Research. Molecular Brain Research·Q L PengB H S Lau
Nov 1, 2002·Brain Pathology·John W OlneyChysanthy Ikonomidou
Nov 19, 2002·Neuroscience Letters·Marieta Barrow HeatonRehae Miller
Nov 5, 2003·Journal of Neuroscience Research·Vinitha RamachandranGeorge Henderson
May 18, 2004·Journal of Neurobiology·Kendra I Siler-MarsiglioMarieta B Heaton

❮ Previous
Next ❯

Citations

Jul 23, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gökçe TanerNurşen Başaran
Jun 23, 2009·Brain Research·Dhara S ShethMary J Druse
Sep 30, 2008·Free Radical Biology & Medicine·Mubeen A AnsariStephen W Scheff
Jun 13, 2014·Phytotherapy Research : PTR·Gökçe TanerNurşen Başaran
Aug 12, 2009·Chemico-biological Interactions·Kehkashan ParveenWaseem A Siddiqui
Jul 22, 2006·Neuroscience Letters·Bo Mi KuHyun Joon Kim
May 4, 2010·Chemico-biological Interactions·Kehkashan ParveenWaseem A Siddiqui
Oct 26, 2016·Neuroscience Letters·Stephen W Scheff, Kelly N Roberts
Sep 25, 2017·Neurotoxicity Research·Jotele Fontana AgostiniEduardo Pacheco Rico
Jul 28, 2010·Alcoholism, Clinical and Experimental Research·Ling LongYing Peng
Jun 16, 2005·Experimental Biology and Medicine·Charles R GoodlettFeng C Zhou
Jan 11, 2018·Translational Neuroscience·Zikang Guo, Jiang Li
Jan 22, 2021·The Cerebellum·Adaze Bijou Enogieru, Oghenakhogie Iroboudu Momodu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis