Protective response of the Ah receptor to ANIT-induced biliary epithelial cell toxicity in see-through medaka

Toxicological Sciences : an Official Journal of the Society of Toxicology
David C VolzDavid E Hinton

Abstract

The adaptive role of the aryl hydrocarbon receptor (Ah receptor or AHR) in protecting against disease-related conditions remains unclear in nonmammalian models, particularly teleosts. Therefore, this study focused on the potential role of AHR in response to biliary epithelial cell toxicity and hepatobiliary alteration in medaka. See-through medaka (STII strain) were exposed for 96 h using the biliary toxicant alpha-naphthylisothiocyanate (ANIT) as a reagent, and fish were evaluated daily using histological and ultrastructural analysis, and by imaging directly through the body wall of living fish. Brightfield and transmission electron microscopy showed that a single ANIT dose (40 mg/kg) specifically induced swelling and apoptosis of bile preductular epithelial cells (BPDECs) as early as 6 h after initial exposure. Following ANIT-induced BPDEC toxicity, in vivo imaging of STII medaka showed significant gallbladder discoloration from 48-72 h. Collectively, these pathologic data suggested that ANIT exposure resulted in acute hepatobiliary changes, lasting < 96 h following initial exposure. We then tested the potential role of AHR in response to ANIT-induced hepatobiliary alteration. Overall, we demonstrated that (1) transient AHR a...Continue Reading

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Citations

Apr 17, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuhua XuJames L Boyer
Dec 15, 2015·Aquatic Toxicology·Melissa ChernickDavid E Hinton
May 9, 2018·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Bao-Long WangZhong-Ze Fang

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