Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

Fish Physiology and Biochemistry
Miaomiao XinOtomar Linhart

Abstract

The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100 μg/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 ± 4% motility and 160 ± 2 μm/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 ± 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1 μg/mL of AFPIII (58 ± 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 ± 6% live cells, while the cryopreserved sperm only contained 26.6 ± 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1 μg/ml of...Continue Reading

References

Apr 1, 1994·Cryobiology·S R PayneG C Upreti
Jan 3, 2003·Cryobiology·Franz LahnsteinerThomas Weismann
Jan 18, 2006·Molecular and Cellular Endocrinology·R John Aitken, Mark A Baker
Dec 18, 2007·Methods in Molecular Biology·Eugeny KopeikaTiantian Zhang
Sep 24, 2008·Fish Physiology and Biochemistry·Ping LiOtomar Linhart
Nov 19, 2008·Biochimica Et Biophysica Acta·Stephen R Wassall, William Stillwell
Jun 12, 2017·Theriogenology·Miaomiao XinOtomar Linhart
Apr 4, 2018·Animal Reproduction Science·Miaomiao XinOtomar Linhart

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Methods Mentioned

BETA
flow cytometry
FACS

Software Mentioned

ISAS
SPSS
FACS Diva

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