Protective role of p21(Waf1/Cip1) against prostaglandin A2-mediated apoptosis of human colorectal carcinoma cells.

Molecular and Cellular Biology
Myriam GorospeN J Holbrook

Abstract

Prostaglandin A2 (PGA2) suppresses tumor growth in vivo, is potently antiproliferative in vitro, and is a model drug for the study of the mammalian stress response. Our previous studies using breast carcinoma MCF-7 cells suggested that p21(Waf1/Cip1) induction enabled cells to survive PGA2 exposure. Indeed, the marked sensitivity of human colorectal carcinoma RKO cells to the cytotoxicity of PGA2 is known to be associated with a lack of a PGA2-mediated increase in p21(Waf1/Cip1) expression, inhibition of cyclin-dependent kinase activity, and growth arrest. To determine if cell death following exposure to PGA2 could be prevented by forcing the expression of p21(Waf1/Cip1) in RKO cells, we utilized an adenoviral vector-based expression system. We demonstrate that ectopic expression of p21(Waf1/Cip1) largely rescued RKO cells from PGA2-induced apoptotic cell death, directly implicating p21(Waf1/Cip1) as a determinant of the cellular outcome (survival versus death) following exposure to PGA2. To discern whether p21(Waf1/Cip1)-mediated protection operates through the implementation of cellular growth arrest, other growth-inhibitory treatments were studied for the ability to attenuate PGA2-induced cell death. Neither serum depletion ...Continue Reading

References

Mar 1, 1992·Experimental Cell Research·A M ChoiN J Holbrook
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Dec 8, 1995·The Journal of Biological Chemistry·T RussoE Appella
Oct 12, 1995·Nature·J BrugarolasG J Hannon
Aug 11, 1995·The Journal of Biological Chemistry·M AkashiH P Koeffler
Mar 1, 1995·Genes & Development·C E CanmanM B Kastan
Mar 9, 1995·Nature·D O Morgan
Oct 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A M ChoiN J Holbrook
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Dec 16, 1993·Nature·Y XiongD Beach
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T D KessisK R Cho
Feb 2, 1996·The Journal of Biological Chemistry·H B LinJ L Hamlin

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Citations

Aug 1, 1997·International Journal of Radiation Biology·N YamagishiH Takebe
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y A WangP Leder
Jan 11, 2000·Molecular and Cellular Biology·W WangM Gorospe
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R Schuette, M C LaPointe
Mar 30, 2001·The Journal of Biological Chemistry·C P FantonR O Pieper
Dec 4, 2003·Journal of Experimental Therapeutics & Oncology·Jae-Won SohI Bernard Weinstein
Dec 26, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Yoshikazu MiwaToshiyuki Sasaguri
Feb 16, 2005·Apoptosis : an International Journal on Programmed Cell Death·Xueli FanJason J Chen
Jun 5, 2008·Neurotoxicity Research·Simone StarkUrsula Felderhoff-Mueser
Apr 23, 2009·International Journal of Cancer. Journal International Du Cancer·Jared M WhitsonRajvir Dahiya
Dec 12, 2012·PloS One·Luca MologniCarlo Gambacorti-Passerini
Jul 24, 2012·Biochemistry. Biokhimii︠a︡·V S RomanovT V Pospelova
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·R MarchesJ W Uhr
May 15, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M K Kang, N H Park
Oct 6, 2007·Medical Oncology·Antonio BrúEduardo López-Collazo
May 28, 1998·The Journal of Biological Chemistry·J ZhuX Chen
Nov 13, 1999·Journal of Virology·J Ciacci-ZanellaC Jones
Dec 22, 1999·The American Journal of Physiology·J HughesS J Shankland
Dec 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Claudia M HattingerRoeland W Dirks
Sep 18, 2001·Journal of Virology·D ChattopadhyayM L Harter
Jan 15, 2005·Antioxidants & Redox Signaling·Michael A O'Reilly
Aug 4, 2005·Annals of Surgical Oncology·Rohit R SharmaWeng-Lang Yang
Mar 9, 2005·The Journal of Biological Chemistry·Yoshimi ArimaHideyuki Saya

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