PMID: 11325541Apr 28, 2001Paper

Protective role of superoxide dismutases against ionizing radiation in yeast

Biochimica Et Biophysica Acta
J H LeeJ W Park

Abstract

The protective role of superoxide dismutases (SODs) against ionizing radiation, which generates reactive oxygen species (ROS) harmful to cellular function, was investigated in the wild-type and in mutant yeast strains lacking cytosolic CuZnSOD (sod1Delta), mitochondrial MnSOD (sod2Delta), or both SODs (sod1Deltasod2Delta). Upon exposure to ionizing radiation, there was a distinct difference between these strains in regard to viability and the level of protein carbonyl content, which is the indicative marker of oxidative damage to protein, intracellular H2O2 level, as well as lipid peroxidation. When the oxidation of 2',7'-dichlorofluorescin was used to examine the hydroperoxide production in yeast cells, the SOD mutants showed a higher degree of increase in fluorescence upon exposure to ionizing radiation as compared to wild-type cells. These results indicated that mutants deleted for SOD genes were more sensitive to ionizing radiation than isogenic wild-type cells. Induction and inactivation of other antioxidant enzymes, such as catalase, glucose 6-phosphate dehydrogenase, and glutathione reductase, were observed after their exposure to ionizing radiation both in wild-type and in mutant cells. However, wild-type cells maintain...Continue Reading

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Citations

Aug 18, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ibrahim KocerKorkmaz Serifoglu
Sep 20, 2008·Biological Trace Element Research·Gao-Yi TanShan-Shan Zheng
Mar 9, 2005·International Journal of Radiation Oncology, Biology, Physics·Meritxell MollàJulián Panés
Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael ChangGrant W Brown
Sep 18, 2002·Antimicrobial Agents and Chemotherapy·Daisuke KobayashiNaoki Watanabe
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·David WunderMira Edgerton
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·N N GesslerT A Belozerskaya
Oct 8, 2013·Antioxidants & Redox Signaling·Aaron K HolleyWilliam H St Clair
Jun 15, 2007·Free Radical Biology & Medicine·Mikael MolinJean Labarre
Mar 29, 2016·ACS Nano·Xiao-Dong ZhangMeixian Li
Oct 8, 2013·Journal of Environmental Radioactivity·Xiaoming ChenXuegang Luo
May 23, 2015·International Journal of Radiation Biology·Shubhankar SumanRakesh Kumar Seth
May 12, 2004·Life Sciences·Yukihisa TakahashiKazuo T Suzuki
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