Protective roles of glutathione in the toxicity of mercury and cadmium compounds to C6 glioma cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M R Cookson, V W Pentreath

Abstract

The neurotoxic effects of mercury (II) chloride, methylmercury (MeHg) and cadmium chloride on astrocytes were modelled using C6 glioma cell cultures. All three compounds were cytotoxic to these cells with an order of potency of cadmium > MeHg > mercurychloride. Addition of reduced glutathione (GSH) to the media protected the cells in all three cases, whereas depletion of GSH with l-buthionine-S,R-sulfoximine enhanced the toxicity of cadmium and mercury chloride but not MeHg. The effects of subcytotoxic concentrations of these compounds on intracellular GSH levels were assessed using chlorobimane staining. All three showed a similar type of effect-an initial depletion of GSH followed by an increase to levels greater than in untreated cells. For mercury chloride-exposed cells (0.37-3.7 muM), the initial depletion occurred over 4 hr with the cells recovering by 7 hr and increases in the GSH content seen after 24 hr. With cadmium or MeHg (0.04-4 muM), the initial depletion was more protracted, with treated cells having less GSH than control cells for 4-7 hr. Glutathione S-transferase activity in the cells was increased after 24 hr of exposure to all three metals to about 200% of control values. These results show that some componen...Continue Reading

References

Feb 1, 1995·Kidney International·H H BohetsP J Dierickx
Feb 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M R CooksonV W Pentreath

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Citations

Sep 1, 2000·Chemico-biological Interactions·N D Slamon, V W Pentreath
Apr 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Mead, V W Pentreath
Mar 1, 2014·Neurotoxicity Research·Hong LiuRichard Salvi
Jul 16, 2010·Environmental Toxicology·Sobhika AgarwalaB S Satish Rao
Jan 1, 2004·Nonlinearity in Biology, Toxicology, Medicine·V W Pentreath, C Mead
Oct 21, 2014·PloS One·Allan Costa-MalaquiasMaría Elena Crespo-Lopez
Aug 25, 2007·The Science of the Total Environment·Claudia GundackerMartin Gencik
Jun 24, 2008·Critical Reviews in Toxicology·Edward J Calabrese
Jan 21, 2006·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Wendy BradfieldAlison Curnow
Jan 1, 2005·Nonlinearity in Biology, Toxicology, Medicine·N Debbie SlamonVic W Pentreath
Feb 24, 2001·Human & Experimental Toxicology·V W Pentreath, N D Slamon
Aug 5, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F Filipak NetoC A Oliveira Ribeiro

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