Protegrin-1 Regulates Porcine Granulosa Cell Proliferation via the EGFR-ERK1/2/p38 Signaling Pathway in vitro.

Frontiers in Physiology
Bo PanJulang Li

Abstract

Antimicrobial peptides (AMPs) are traditionally known to be essential components in host defense via their broad activities against bacteria, fungi, viruses, and protozoa. Their immunomodulatory properties have also recently received considerable attention in mammalian somatic tissues of various species. However, little is known regarding the role of AMPs in the development and maturation of ovarian follicles. Protegrin-1 (PG-1) is an antimicrobial peptide which is known to have potent antimicrobial activity against both gram positive and negative bacteria. Here we report that the PG-1 is present in the porcine ovarian follicular fluid. Treatment of granulosa cell with PG-1 enhanced granulosa cell proliferation in a dose-dependent manner. This is accompanied by increased expression of cell-cycle progression-related genes such as cyclin D1(CCND1), cyclin D2 (CCND2), and cyclin B1(CCNB1). Additionally, Western blot analysis showed that PG-1 increased phosphorylated epidermal growth factor receptor (EGFR), and the phosphorylated-/total extracellular signal-regulated kinase (ERK)1/2 ratio. Pretreatment with either U0126, a specific ERK1/2 phosphorylation inhibitor, or EGFR kinase inhibitor, AG1478, blocked the PG-1 induced prolifer...Continue Reading

References

Jan 1, 1991·International Review of Cytology·A N Hirshfield
May 24, 1984·Nature·M B MathewsJ I Garrels
Feb 1, 1994·Microscopy Research and Technique·F Kotsuji, T Tominaga
Feb 1, 1994·Biology of Reproduction·J E Fortune
Aug 7, 1997·Journal of Immunological Methods·O SørensenN Borregaard
Oct 9, 1999·The Journal of Biological Chemistry·F BeierP LuValle
Feb 24, 2000·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·T MaruoH Matsuo
Jan 16, 2002·Current Opinion in Genetics & Development·Claire R Weston, Roger J Davis
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 25, 2003·Biochemical and Biophysical Research Communications·Dhara VaishnavSubbiah Pugazhenthi
Oct 4, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Raymond J RodgersDarryl L Russell
Jul 1, 2004·American Journal of Physiology. Renal Physiology·Jing DongTerrence J Monks
Jan 14, 2005·International Journal of Clinical Practice·C H GoliasK Charalabopoulos
Oct 21, 2005·Trends in Biochemical Sciences·Marcos Malumbres, Mariano Barbacid
Dec 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gary K Schwartz, Manish A Shah
Aug 8, 2007·Cellular Signalling·Roza ZandiHans Skovgaard Poulsen
Jan 30, 2008·Journal of Assisted Reproduction and Genetics·S DasR Gazvani
Jul 2, 2010·Endocrinology·Oliver R OakleyChemyong Ko
May 24, 2011·Trends in Endocrinology and Metabolism : TEM·Oliver R OakleyCheMyong Ko
May 10, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Robert Roskoski
Apr 19, 2013·Nature Reviews. Molecular Cell Biology·Niels MailandSimon Bekker-Jensen
May 28, 2013·Journal of Proteomics·Aditi S AmbekarSrabani Mukherjee
May 30, 2013·Indian Journal of Dermatology·Xinhong GeJianzhong Zhang
Feb 14, 2014·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Shubhadeep RoychoudhuryAdriana Kolesarova
Sep 22, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Mark M KushnirJonas Bergquist
May 21, 2016·JBRA Assisted Reproduction·Laís Basuino, Carolina F Silveira
Jul 1, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Ivana KnyghtDavid J Barlow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.