PMID: 6172466Nov 1, 1981Paper

Protein A, avidin, and biotin in immunohistochemistry

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
S M Hsu, L Raine

Abstract

Conjugated and unlabeled peroxidase antibody methods have proven to be quite satisfactory in localizing sites of antigen-antibody reaction. The use of avidin-biotin-peroxidase complex (ABC), as well as protein A, can contribute significantly to the field of immunohistochemistry. The sensitivity and specificity of several immunohistochemical methods is compared. In general, the ABC method produced the most intense staining and the least background staining of any method tested. The unlabeled antibody (peroxidase-antiperoxidase: PAP) method also yielded satisfactory results, but it was less intense than the ABC method. In comparison to the PAP method, the indirect conjugated method presented slightly inferior staining intensities and significantly higher background staining. Protein A techniques produced a range of staining sensitivities similar to or inferior to the PAP technique. The main disadvantage in using protein A is that it reacts with intrinsic immunoglobulin (Ig) G, thus producing an intense background. Therefore, its use is not recommended on tissues that have either abundant immunoglobulins in their interstitium or numerous IgG-containing plasma cells.

Citations

Feb 5, 1996·The Journal of Comparative Neurology·S S SpicerB A Schulte
Mar 13, 1998·The Journal of Pathology·H HagerJ Gliemann
Jun 22, 2000·The Journal of Comparative Neurology·E ComoliN S Canteras
Sep 7, 2000·The Journal of Comparative Neurology·L LeventhalS Palfi
Jan 1, 1990·The American Journal of Anatomy·S S SpicerB A Schulte
Jan 1, 1992·American Journal of Industrial Medicine·S DiniV Sforza
Aug 15, 2001·The Journal of Comparative Neurology·M GotoN S Canteras
Jul 17, 2001·Biochemical and Biophysical Research Communications·M K AhlrothM S Kulomaa
Oct 19, 2001·Biochemical and Biophysical Research Communications·M K AhlrothM S Kulomaa
Nov 16, 2001·Biochemical and Biophysical Research Communications·M NakamuraI Kumagai
Jan 1, 1991·Histochemistry·M CastellucciR Chiquet-Ehrismann
Jan 1, 1983·Archives of Dermatological Research·E BertiR Caputo
Feb 1, 1988·Biochemical Genetics·P W MamulaR C Karn
Jan 1, 1984·Acta Neuropathologica·G P WangH M Wisniewski
Jan 1, 1989·Virchows Archiv. A, Pathological Anatomy and Histopathology·S M ZhangX Z Zhang
Jan 1, 1986·Histochemistry·F Sánchez-FrancoL Muñoz Barragan
Mar 1, 1983·The Histochemical Journal·F T Bosman
Apr 1, 1987·Cell and Tissue Research·O NilssonL E Ericson
May 1, 1987·The Histochemical Journal·C J JonesR W Stoddart
May 2, 2003·European Journal of Nuclear Medicine and Molecular Imaging·Silvana Del VecchioMarco Salvatore
Sep 1, 2006·Cancer Chemotherapy and Pharmacology·R F C LeitãoG A C Brito
Jul 28, 2010·Cancer Chemotherapy and Pharmacology·C A C X MedeirosG A C Brito
Apr 7, 2011·Inflammation·Francisco Yuri Bulcão MacedoRonaldo A Ribeiro

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