Protein A chromatography: Challenges and progress in the purification of monoclonal antibodies

Journal of Separation Science
Ana Mayela Ramos-de-la-PeñaOscar Aguilar

Abstract

Antibodies for therapeutic use are being continuously approved and their demand has been steadily growing. As known, the golden standard for monoclonal antibody (mAb) purification is Protein A affinity chromatography, a technology that has gained high interest because of its great performance and capabilities. The main concerns are the elevated resins costs and their limited lifetime compared to other resins (e.g. ion exchange chromatography). Great efforts have been carried out to improve purification conditions, such as resin characterization and designing alkali/acid stable resins with a longer lifetime. Modification of Protein A ligands and alternative formats such as monoliths membranes and microshperes have been tested to increase the purification performance. New technology has been proposed to improve the large-scale separation; in addition, alternative ligands have been suggested to capture mAbs instead of Protein A ligand; however, most of the information is locked by pharmaceutical companies. This mini review summarizes and describes the advances, results, and impact on the Protein A chromatography purification processing.

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Citations

May 31, 2020·International Journal of Molecular Sciences·Annalisa BarozziStefano Menegatti
Dec 28, 2019·Membranes·Eleonora LalliGiulio C Sarti
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Nagesh K Tripathi, Ambuj Shrivastava
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Jun 25, 2020·Biotechnology Journal·Raquel Dos SantosAna Cecília A Roque
Dec 29, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Magnus WetterhallGustav Rodrigo
Apr 8, 2021·Protein Expression and Purification·Claudia DurantiAnnarosa Arcangeli
Jun 19, 2021·Engineering in Life Sciences·Liming Huan, Qing-Hong Shi
Aug 2, 2021·Cancer Cell International·Gang ChengShibing Wang
Oct 29, 2021·Frontiers in Microbiology·Matthew J McNultyKaren A McDonald

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