PMID: 9421974Jan 9, 1998Paper

Protein-A-mediated targeting of bacteriochlorophyll-IgG to Staphylococcus aureus: a model for enhanced site-specific photocytotoxicity

Photochemistry and Photobiology
S GrossY Salomon

Abstract

A model for studying the efficiency of photodynamic action with a photosensitizer placed exclusively on the bacterial cell wall has been used. Bacteriochlorophyllide molecules, conjugated to rabbit immunoglobulin G (IgG), were synthesized. The conjugated pigment bacteriochlorophyll (Bchl)-IgG bound with high specificity to protein-A residues naturally exposed on the cell wall of the bacterium Staphylococcus aureus Cowan I. In bacterial suspensions the phototoxicity of the targeted conjugates (0.5-2.5 pigment per IgG molecule) was dose dependent (LD50 = 1.7 microM) in the presence of light (lambda > 550 nm) and inhibited by native IgG but not by ovalbumin, suggesting selective interaction with protein-A on the bacterial cell wall. No dark toxicity was noticed even with the highest conjugate concentration tested. In contrast, the photocytotoxicity of bacteriochlorophyll-serine (Bchl-Ser, LD50 = 0.07 microM) used as a nontargeted control was not inhibited by IgG. In spite of its lower apparent potency, Bchl-IgG was found to be 30 times more efficacious than Bchl-Ser: At LD50, only 66,000 Bchl-IgG molecules were bound per bacterium compared to 1,900,000 molecules of Bchl-Ser. The higher efficacy of Bchl-IgG is explained by its excl...Continue Reading

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Citations

Jan 7, 2004·Advanced Drug Delivery Reviews·G A M S van DongenM B Vrouenraets
May 10, 2005·Journal of Photochemistry and Photobiology. B, Biology·Peter Meisel, Thomas Kocher
Jun 6, 2000·Advanced Drug Delivery Reviews·O V GerasimovD H Thompson
Dec 1, 2005·Photodiagnosis and Photodynamic Therapy·Katie O'RiordanTayyaba Hasan
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Michael R Hamblin, Tayyaba Hasan
Jul 17, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Michelle L EmbletonMichael Wilson
Dec 8, 2010·Periodontology 2000·Nikolaos S Soukos, J Max Goodson
Oct 6, 2004·International Journal of Immunopathology and Pharmacology·T N Demidova, M R Hamblin
Dec 25, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rebecca V VinceJohn Greenman
Mar 17, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aaron J BullousRoss W Boyle
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark D Savellano, Tayyaba Hasan
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chih-Hui YangJei-Fu Shaw
Apr 28, 2005·Journal of the American Chemical Society·Yahel Vakrat-HagliliAvigdor Scherz

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