PMID: 6979776Mar 1, 1982Paper

Protein A reactivity of lymphocytes from some patients with chronic lymphocytic leukaemia mediated by an interaction with the F(ab')2 region of surface immunoglobulin

Scandinavian Journal of Immunology
S RomagnaniM Ricci

Abstract

Peripheral blood lymphocytes (PBL) from 15 of 38 patients with chronic lymphocytic leukaemia (CLL) were capable of forming rosettes with human erythrocytes coated with staphylococcal protein A (SpA-HRBC). PBL from seven patients also showed a marked proliferative response after stimulation with Staphylococcus aureus bacteria strain Cowan I (Cowan Staph). The SpA-rosetting of CLL cells was inhibited by incubation with F(ab')2 fragments of anti-immunoglobulin (Ig) antibodies. In addition, incubation with these fragments inhibited the proliferative response of leukaemic B cells to Cowan Staph. The Cowan-Staph induced proliferation of CLL cells was also impaired by the addition of normal human IgG and human IgG F(ab')2 fragments to the cultures, whereas non-immune rabbit IgG was not inhibitory. The inhibitory activity of human IgG F(ab')2 fragments was retained by a SpA-Sepharose column, and it was found in the material recovered from the same column by acid elution. These data indicate that an interaction between SpA and a structure located in the F(ab')2 region of surface Ig of different classes is responsible for either SpA-binding or the Cowan-Staph-induced proliferative response of PBL from some patients with CLL.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C L Sidman, E R Unanue
Jan 1, 1978·Scandinavian Journal of Immunology·G Banck, A Forsgren
Mar 1, 1978·The Journal of Experimental Medicine·D G SieckmannW E Paul
Mar 1, 1976·European Journal of Immunology·A ForsgrenH Wigzell

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Citations

Jan 28, 2014·International Journal of Medical Microbiology : IJMM·Barbara M BrökerIsabelle Bekeredjian-Ding
Jul 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lindsay N DeisTerrence G Oas
Nov 23, 1984·Biochimica Et Biophysica Acta·T C GentileR M Watt

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