Protein A reactivity of various mammalian immunoglobulins
Serum samples and immunoglobulin fractions of eight mammalian species were applied to a Sepharose--protein A column. As with the human immunoglobulin subclasses IgG1, IgG2 and IgG4, all examined IgG classes and subclasses were bound to a greater or lesser extent to protein A. However, the binding of IgG1 of ruminants was very poor. Polyclonal IgM and IgA of the pig, the dog and the cat may be separated in protein A reactive and protein A non-reactive fractions. In addition, monoclonal canine IgM and IgA partially reacted with protein A. In combination with methods such as ammonium sulphate precipitation, ion exchange chromatography and gel-filtration, affinity chromatography with protein A is recommended for the rapid purification of certain Ig (sub)classes of a number of mammalian species.
Comparative study of procedures for histological detection of lectin binding by use of Griffonia simplicifolia agglutinin I and gastrointestinal mucosa of the rat
Radioimmunochemical methods for the quantitative autoradiographic determination of antigens in brain and other tissues
Concentration of amylase along its secretory pathway in the pancreatic acinar cell as revealed by high resolution immunocytochemistry
A comparison of immunoferritin, immuno-enzyme and gold-labelled protein A methods for the localization of capsular antigen on frozen thin sections of the bacterium, Pasteurella haemolytica
Vaccination against Boophilus microplus: localization of antigens on tick gut cells and their interaction with the host immune system
Identification of lactate dehydrogenase-X translated in vitro from mouse testicular poly A-containing mRNA
Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues
Development of a solid-phase radioimmunoassay for antibody to antigens of Babesia bovis infected bovine erythrocytes
Enzyme-linked immunosorbent assays (ELISAs) for the determination of contaminants resulting from the immunoaffinity purification of recombinant proteins
Optimization of binding capacity and specificity of protein G on various solid matrices for immunoglobulins
Purification of amatoxin-specific antibodies from rabbit sera by affinity chromatography, their characterization and use in toxicological studies
Interaction of bovine immunoglobulin gamma 2 (IgG2) with staphylococcal protein A: evidence for IgG2a and IgG2b sub-subclasses in the bovine
Differences in IgG1 and IgG2 responses of cattle infected with Coxiella burnetii and following vaccination
Simultaneous isolation of intestinal IgA and IgG from rabbits infected intraduodenally with Vibrio cholerae 01 by combined lectin affinity chromatography involving jacalin and protein A
Staphylococcal protein A purification of rodent IgG1 and IgG2 with particular emphasis on syrian hamsters
The immunochemistry of sandwich ELISAs--V. The capture antibody performance of polyclonal antibody-enriched fractions prepared by various methods
Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique
Enzyme linked immunoassay and fluorescent antibody techniques in the diagnosis of viral diseases using staphylococcal protein-A instead of anti-gamma-globulins
Optimum conditions for the radioimmunoprecipitation assay for the major internal protein of bovine leukemia virus
A radiolabeled staphylococcal protein A assay for detection of anti-erythrocyte IgG in warm agglutinin autoimmune hemolytic anemia of dogs and man
Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay
Dominance of Chlamydia psittaci-specific IgG2 subclass in the humoral immune responses of naturally and experimentally infected cattle
Detection of IgM rheumatoid factor in canine serum using a standardized enzyme-linked immunosorbent assay
Purification and characterization of IgG1 and IgG2 from buffalo (Bubalus bubalis) serum and colostrum
Preparation of anti-canine serum amyloid A (SAA) serum and purification of SAA from canine high-density lipoprotein
A radioimmunohistochemical method for autoradiographic visualization of cell antigens using 125I-staphylococcal protein A
Isolation and characterization of the glucose-6-phosphate dehydrogenase gene of Drosophila melanogaster
Production of Brucella abortus-specific protein A-reactive antibodies (IgG2) in infected and vaccinated cattle
Antibody isotype responses in the serum and respiratory tract to primary and secondary infections with equine influenza virus (H3N8)
New methodology for the obtainment of antibothropic factors from the South American opossum (Didelphis marsupialis) and jararaca snake (Bothrops jararaca)
Biochemical characterization of canine blood group antigens: immunoprecipitation of DEA 1.2, 4 and 7 and identification of a dog erythrocyte membrane antigen homologous to human Rhesus
Direct immunoassay for detecting Escherichia coli colonies that contain polypeptides encoded by cloned DNA segments
cDNA clones for the heavy chain of HLA-DR antigens obtained after immunopurification of polysomes by monoclonal antibody
Intramitochondrial movement of adrenal sterol carrier protein with cholesterol in response to corticotropin
A study on the synthesis and secretion of immunoglobulins by the Jarderian gland of the fowl after eyedrop vaccination against infectious bronchitis at 1-day-old
Depressed degranulation response of synovial fluid polymorphonuclear leucocytes from patients with rheumatoid arthritis to IgG aggregates
Use of FITC-protein A in place of fluorescein-conjugated anti-gammaglobulins for rapid virus diagnosis by immunofluorescence
Human eosinophil arylsulfatase B. Structure and activity of the purified tetrameric lysosomal hydrolase
A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients
Determination of C-reactive protein in serum and plasma from healthy dogs and dogs with pneumonia by ELISA and slide reversed passive latex agglutination test
A new immunoenzyme tracer for immunohistology: peroxidase-labeled protein A. Its application for determination of antinuclear antibodies
Detection of antibodies to myelin basic protein by solid-phase radioimmunoassay with [125I]protein A
Antigenic variation between three strains of rinderpest virus detected by kinetic neutralisation and competition ELISA using early rabbit antisera
Cerebrospinal fluid and serum oligoclonal IgG bands in rabbits with experiment allergic encephalomyelitis
Two-step purification of a murine monoclonal antibody intended for therapeutic application in man. Optimisation of purification conditions and scaling up
What's new in light and electron microscopic immunocytochemistry? Application of the protein A-gold technique to routinely processed tissue
Bottlenose dolphin (Tursiops truncatus) papillomaviruses: vaccine antigen candidates and screening test development
Dirofilaria immitis: performance and standardization of specific antibody immunoassays for filariasis
Comparative quantification of rat brain and pituitary angiotensin-converting enzyme with autoradiographic and enzymatic methods
Rapid and sensitive detection of dengue viral antigen using immunogold in light microscopy and solid phase gold immunoassay (SPGIA)
Enzyme-linked immunosorbent assay (ELISA) using staphylococcal protein A for the measurement of rabies antibody in various species
Ex vivo perfusion of plasma over protein A columns in human mammary adenocarcinoma. Role of the Fc-binding capacity of protein A in the side effects and the tumoricidal response
A microplate enzyme-linked immunosorbent assay for measuring antibody to Anaplasma marginale in cattle serum
Non-immune Fab- and Fc- mediated interactions of avian Ig with S. aureus and group C and G streptococci
A new immunoenzyme tracer for localization of antibodies in immunohistology: peroxidase-labeled protein A
A simple two-step immunocytochemical method using protein A-peroxidase to stain immunoreactive cell antigens
Influence of endogenous cortisol on eosinophil function in sensitized pigs: direct measurements of eosinophil peroxidase
Effect of anticardiolipin antibody in patients with recurrent fetal loss on thrombomodulin-dependent protein C activation
Oxidative response of polymorphonuclear leucocytes to synovial fluids from patients with rheumatoid arthritis
Immunohistochemical distribution of heart-type fatty acid-binding protein immunoreactivity in normal human tissues and in acute myocardial infarct
Carbonic anhydorase isoenzyme I (CA-I) concentration in feces and urine as a temporary marker of occult blood in beagle dogs
Detection of anti-Leishmania immunoglobulin G antibodies in urine specimens of dogs with leishmaniasis
Genetic variations in immunoglobulin G3 and association with staphylococcal intra-mammary infections in cattle and buffaloes
Soluble complex formation of bovine immunoglobulin G2 with staphylococcal protein A studied by gel filtration chromatography
Chromogenic immunodetection of human serum albumin and alpha-L-fucosidase clones in a human hepatoma cDNA expression library
Echinococcus multilocularis: variation among samples of cyst fluid in binding of parasite-specific antibodies
Acquired canine myasthenia gravis: immunocytochemical localization of immune complexes at neuromuscular junctions
Isolation, purification and characterization of intracellular calmodulin like protein (CALP) from Mycobacterium phlei
Natural autoantibodies to the gonadotropin-releasing hormone receptor in polycystic ovarian syndrome.
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Evolution of Pluripotency
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Lipidomics & Rhinovirus Infection
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Chronic Fatigue Syndrome
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Cell Atlas Along the Gut-Brain Axis
Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.