Protein Acetylation Is Involved in Salmonella enterica Serovar Typhimurium Virulence

The Journal of Infectious Diseases
Yu SangYufeng Yao

Abstract

Salmonella causes a range of diseases in different hosts, including enterocolitis and systemic infection. Lysine acetylation regulates many eukaryotic cellular processes, but its function in bacteria is largely unexplored. The acetyltransferase Pat and NAD(+)-dependent deacetylase CobB are involved in the reversible protein acetylation in Salmonella Typhimurium. Here, we used cell and animal models to evaluate the virulence of pat and cobB deletion mutants in S. Typhimurium and found that pat is critical for bacterial intestinal colonization and systemic infection. Next, to understand the underlying mechanism, genome-wide transcriptome was analyzed. RNA sequencing data showed that the expression of Salmonella pathogenicity island 1 (SPI-1) is partially dependent on pat In addition, we found that HilD, a key transcriptional regulator of SPI-1, is a substrate of Pat. The acetylation of HilD by Pat maintained HilD stability and was essential for the transcriptional activation of HilA. Taken together, these results suggest that a protein acetylation system regulates SPI-1 expression by controlling HilD in a posttranslational manner to mediate S. Typhimurium virulence.

References

Nov 1, 1985·The Journal of Infectious Diseases·C E HormaecheH S Joysey
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·J LiR K Selander
Aug 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M A SteinB B Finlay
Sep 19, 1997·Trends in Microbiology·E A Groisman, H Ochman
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·R RamakrishnanR B Bourret
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Sep 24, 1998·Infection and Immunity·A J BäumlerL G Adams
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·D HershA Zychlinsky
Jul 10, 1999·Clinical Microbiology Reviews·K H Darwin, V L Miller
Apr 1, 2000·Microbes and Infection·S L MarcusB B Finlay
Jun 9, 2000·Molecular Microbiology·T S Wallis, E E Galyov
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J E Galán
Feb 8, 2005·Current Opinion in Microbiology·Jayesh C Patel, Jorge E Galán
Jul 17, 2007·Biotechnology and Applied Biochemistry·Valiollah BabaeipourFatemeh Tabandeh
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Víctor H BustamanteJosé L Puente
Sep 3, 2014·Molecular & Cellular Proteomics : MCP·Fengying LiuFeng Ge
Oct 24, 2014·Frontiers in Immunology·Daniel HurleyMarta Martins
Dec 18, 2014·Molecular Systems Biology·Sara Castaño-CerezoManuel Cánovas
Jun 14, 2015·Applied and Environmental Microbiology·Jie RenYu-Feng Yao

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Citations

May 26, 2016·Journal of Proteomics·Takfarinas KentacheJulie Hardouin
Sep 21, 2016·Applied Microbiology and Biotechnology·Vicente BernalManuel Cánovas
Apr 26, 2017·Journal of Bacteriology·Valerie J Carabetta, Ileana M Cristea
Aug 26, 2018·Applied and Environmental Microbiology·Kalle LiimattaAlexandra E Purdy
Jun 19, 2018·Frontiers in Microbiology·Yangying SunDaodong Pan
May 16, 2019·Annual Review of Microbiology·Chelsey M VanDrisse, Jorge C Escalante-Semerena
Apr 17, 2020·Microbiology and Molecular Biology Reviews : MMBR·Rachel M Burckhardt, Jorge C Escalante-Semerena
May 6, 2017·Frontiers in Microbiology·Shuxian LiYu-Feng Yao
Sep 21, 2018·Frontiers in Microbiology·Sébastien Bontemps-GalloFrank C Gherardini
Nov 18, 2020·The Journal of Microbiology·Hyojeong KooJung-Shin Lee
Nov 6, 2020·Science Progress·Suzanne Forrest, Martin Welch
Dec 16, 2020·Infection and Immunity·Jianhui LiYu-Feng Yao
Sep 24, 2020·Journal of Proteome Research·Valerie J Carabetta
Apr 23, 2019·Journal of Proteome Research·Xu Zhang, Daniel Figeys
Nov 23, 2021·Frontiers in Microbiology·Ayesha NisarMingxiong He

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