Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates

Journal of Biotechnology
Natalia S de Groot, Salvador Ventura

Abstract

Recent data show that protein aggregation as bacterial inclusion bodies does not necessarily imply loss of biological activity. Here, we investigate the effect of a large set of single-point mutants of an aggregation-prone protein on its specific activity once deposited in inclusion bodies. The activity of such aggregates significantly correlates with the predicted aggregation rates for each mutant, suggesting that rationally tuning the kinetic competition between folding and aggregation might result in highly active, inclusion bodies. The exploration of this technology during recombinant protein production would have a significant biotechnological value.

References

Apr 13, 2000·FEBS Letters·M M CarrióA Villaverde
Aug 15, 2003·Nature·Fabrizio ChitiChristopher M Dobson
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic

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Citations

Dec 2, 2009·Applied and Environmental Microbiology·Ana Belén CampeloBeatriz Martínez
Jan 1, 2013·Microbial Cell Factories·Anna Villar-Piqué, Salvador Ventura
May 5, 2012·Microbial Cell Factories·Anna Villar-PiquéSalvador Ventura
May 26, 2012·Microbial Cell Factories·Mirjana LiovicTina Zupancic
Jun 1, 2010·Microbial Cell Factories·Britta JürgenPeter Neubauer
Nov 3, 2010·Microbial Cell Factories·Elena García-Fruitós
Mar 3, 2010·PloS One·Natalia S de Groot, Salvador Ventura
Jul 25, 2008·Future Microbiology·Natalia S de GrootSalvador Ventura
Jan 16, 2014·BMC Biotechnology·Thorny Cecilie Bie AndersenAnne Spurkland
Oct 9, 2008·FEBS Letters·Alba EspargaróSalvador Ventura
Nov 15, 2006·FEBS Letters·Natalia Sánchez de Groot, Salvador Ventura
Nov 19, 2015·Biotechnology Journal·Anna Villar-PiquéRaimon Sabate
Aug 4, 2009·Trends in Biochemical Sciences·Natalia S de GrootSalvador Ventura
Sep 2, 2008·Journal of Biotechnology·Gerd MargreiterKarl Bayer
Oct 9, 2007·Journal of Molecular Biology·Elena García-FruitósAntonio Villaverde
Nov 5, 2008·Biotechnology and Bioengineering·Mónica Martínez-AlonsoAntonio Villaverde
Dec 28, 2011·Journal of Molecular Biology·Anna Villar-PiqueSalvador Ventura
Jan 30, 2014·Biochimica Et Biophysica Acta·Susanna NavarroSalvador Ventura
Jun 15, 2007·FEMS Microbiology Letters·Mónica Martínez-AlonsoAntonio Villaverde
Jul 14, 2017·Preparative Biochemistry & Biotechnology·Cornelia WaltherAstrid Dürauer
Oct 3, 2006·Biotechnology and Bioengineering·Andrea VeraAntonio Villaverde
Jul 11, 2007·Nature Biotechnology·Nuria González-MontalbánAntonio Villaverde
Mar 30, 2019·Current Protein & Peptide Science·Ana B CaballeroRaimon Sabate
Jan 13, 2012·Chembiochem : a European Journal of Chemical Biology·Garrick F TaylorPhilip T F Williamson
Jun 4, 2020·Microbial Cell Factories·Marcos Gil-GarciaSalvador Ventura
Jul 24, 2013·Bioconjugate Chemistry·Anika C JahnsBernd H A Rehm
Jul 24, 2018·Molecular Pharmaceutics·Marcos Gil-GarciaSalvador Ventura

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