Protein adsorption and bacterial adhesion to biliary stent materials

The Journal of Surgical Research
J L YuA Ljungh

Abstract

Four biliary stents inserted for relief of jaundice in patients with biliary obstruction due to carcinoma of pancreatic head were examined for the adsorption of biliary proteins and bacterial colonization. Fibronectin and vitronectin (S-protein) were found to be the two main proteins adsorbed on the inner surface of the stents. Biliary isolates included Escherichia coli, Enterococcus faecalis, and Candida albicans. In vitro studies were performed to clarify the kinetics of biliary protein and bacterial adhesion. Biliary drains of polytetrafluorethylene, polyethylene, polyurethane, and rubber were placed in a flow cell and perfused with human bile at 37% C for 24 hr. The materials were subjected to either detection of adsorbed biliary proteins or perfusion with 3 H-labeled E. coli cells (1 X 10 6 cfu/ml). The results show that the adsorbed biliary proteins were detectable on the surface of biliary stents and able to enhance bacterial adhesion to the surface in the first 24 hrs after the exposure of stent materials to bile, and that both the adsorption of biliary proteins and the adhesion of bacterial cells were material- and strain-dependent. Furthermore, there was a clear correlation between the amount of adsorbed fibronectin a...Continue Reading

Citations

Aug 7, 2004·Journal of Clinical Microbiology·Gianfranco DonelliBruna Facinelli
Apr 26, 2007·Clinical Medicine & Research·Gianfranco DonelliAntonio Basoli
Jun 21, 2011·Gastrointestinal Endoscopy Clinics of North America·Inge Huibregtse, Paul Fockens
Jun 22, 2007·Transfusion·Carey GrecoSandra Ramírez-Arcos
Mar 24, 2016·Clinical Endoscopy·Chang-Il Kwon, Glen A Lehman
Mar 12, 2005·World Journal of Gastroenterology : WJG·Markus-K DienerHelmut Friess
Jul 1, 2004·Journal of Biomedical Materials Research. Part a·Ryo MaeyamaTakehisa Matsuda
Mar 15, 2006·Current Gastroenterology Reports·D Nageshwar ReddyOng Wai Choung
Jun 6, 2020·Khirurgiia·A V ShabuninA A Karpov
Jan 24, 2018·World Journal of Gastroenterology : WJG·Chetana VaishnaviRakesh Kochhar
Mar 12, 2009·Journal of Clinical Gastroenterology·Torsten HerzogWaldemar Uhl
Mar 16, 2021·Bio-medical Materials and Engineering·Akane YamabeMakoto Iijima

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