Protein adsorption mechanisms at rough surfaces: Serum albumin at a gold substrate

Journal of Colloid and Interface Science
Zbigniew AdamczykAndrzej Bernasik

Abstract

Adsorption kinetics of human serum albumin (HSA) at a gold substrate was studied using the quartz microbalance (QCM), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). Measurements were performed at pH 3.5 for various bulk suspension concentrations and ionic strengths. The QCM experimental data were compared with the dry coverage of HSA derived from AFM and from the solution of the mass transfer equation acquired using a hybrid random sequential adsorption model. In this way, the water factor and the dynamic hydration function for HSA monolayers were quantitatively evaluated as a function of dry coverage for various ionic strengths. A comparison of these results with previously known for a silica sensor confirmed that the QCM kinetic measurements are sensitive to the roughness of the sensor characterized in terms of the rms parameter. For the more rough gold sensor (rms = 2.5 nm, average surface feature size 6 nm) the QCM mass transfer rate constant was 2.6 times lower than for the silica sensor characterized by rms = 0.86 nm. This gives for the gold sensor the apparent water factor equal to one and zero hydration function. Moreover, the hydration function increased for larger HSA coverage and was depend...Continue Reading

Citations

Mar 8, 2019·Physical Chemistry Chemical Physics : PCCP·Maria MorgaZbigniew Adamczyk
Jan 14, 2021·Nanomaterials·Anna Bratek-SkickiZbigniew Adamczyk
Nov 26, 2020·Colloids and Surfaces. B, Biointerfaces·Małgorzata Nattich-RakZbigniew Adamczyk
Jan 23, 2021·Journal of Colloid and Interface Science·Mohammadhasan HedayatiMatt J Kipper
Jun 3, 2021·International Journal of Environmental Research and Public Health·Monika WasilewskaZbigniew Adamczyk
Jul 21, 2021·International Journal of Biological Macromolecules·Hong-Yu TaoYong-Su Zhen
Aug 9, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Monika WasilewskaMichał Cieśla
Aug 25, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Athénaïs DavantèsDenis Renard

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