Protein aggregate turbidity: Simulation of turbidity profiles for mixed-aggregation reactions

Analytical Biochemistry
Damien HallJohn A Carver

Abstract

Due to their colloidal nature, all protein aggregates scatter light in the visible wavelength region when formed in aqueous solution. This phenomenon makes solution turbidity, a quantity proportional to the relative loss in forward intensity of scattered light, a convenient method for monitoring protein aggregation in biochemical assays. Although turbidity is often taken to be a linear descriptor of the progress of aggregation reactions, this assumption is usually made without performing the necessary checks to provide it with a firm underlying basis. In this article, we outline utilitarian methods for simulating the turbidity generated by homogeneous and mixed-protein aggregation reactions containing fibrous, amorphous, and crystalline structures. The approach is based on a combination of Rayleigh-Gans-Debye theory and approximate forms of the Mie scattering equations.

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Citations

Aug 2, 2016·Analytical Biochemistry·Roshan C DalpadadoDamien Hall
Aug 11, 2016·International Journal of Biological Macromolecules·Andrea StirpeRita Guzzi
Jun 14, 2018·Biopolymers·D GalanteC D'Arrigo
Sep 27, 2018·Chemical Communications : Chem Comm·Ram P GokulaArindam Chowdhury
Jul 23, 2020·Protein Science : a Publication of the Protein Society·Calvin J VetterKirsten J Lampi
Dec 23, 2016·Biophysics Reviews·Ran ZhaoDamien Hall
Oct 16, 2020·Protein Science : a Publication of the Protein Society·Seyed Hamidreza ArabiDariush Hinderberger

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