Protein aggregates encode epigenetic memory of stressful encounters in individual Escherichia coli cells

PLoS Biology
Sander K GoversAbram Aertsen

Abstract

Protein misfolding and aggregation are typically perceived as inevitable and detrimental processes tied to a stress- or age-associated decline in cellular proteostasis. A careful reassessment of this paradigm in the E. coli model bacterium revealed that the emergence of intracellular protein aggregates (PAs) was not related to cellular aging but closely linked to sublethal proteotoxic stresses such as exposure to heat, peroxide, and the antibiotic streptomycin. After removal of the proteotoxic stress and resumption of cellular proliferation, the polarly deposited PA was subjected to limited disaggregation and therefore became asymmetrically inherited for a large number of generations. Many generations after the original PA-inducing stress, the cells inheriting this ancestral PA displayed a significantly increased heat resistance compared to their isogenic, PA-free siblings. This PA-mediated inheritance of heat resistance could be reproduced with a conditionally expressed, intracellular PA consisting of an inert, aggregation-prone mutant protein, validating the role of PAs in increasing resistance and indicating that the resistance-conferring mechanism does not depend on the origin of the PA. Moreover, PAs were found to confer r...Continue Reading

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Citations

Oct 22, 2019·FEMS Microbiology Reviews·Frederic D SchrammKristina Jonas
Mar 10, 2020·PLoS Genetics·Laasya SamhitaDeepa Agashe
May 24, 2019·PLoS Biology·Audrey Menegaz ProencaLin Chao
Feb 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·David W BastaDianne K Newman
Mar 3, 2020·Frontiers in Microbiology·Hui LiMichael G Gänzle
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Mar 2, 2019·Current Genetics·Julien MortierAbram Aertsen
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Jan 26, 2021·Evolution; International Journal of Organic Evolution·Laasya SamhitaDeepa Agashe
Dec 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tamar SzokeOrna Amster-Choder
Feb 8, 2021·Current Opinion in Cell Biology·Yanfei Jiang, Nan Hao
Oct 30, 2020·Microbiology and Molecular Biology Reviews : MMBR·Alexander Cambré, Abram Aertsen
Mar 25, 2021·Nature Communications·Ingo WohlgemuthMarina V Rodnina
May 4, 2021·Frontiers in Molecular Biosciences·Celien BollenJan Michiels
Jun 24, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kevin S FarquharDaniel A Charlebois
Jul 6, 2021·Frontiers in Cell and Developmental Biology·Ulrich Karl Steiner
Aug 17, 2021·PLoS Pathogens·Edgar E LlontopChuck S Farah

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Methods Mentioned

BETA
protein folding
fluorescence microscopy
PCR

Software Mentioned

Nikon
NIS
ImageJ
elements
R
Oufti
MicrobeJ
MicrobeTracker
SpotFinder

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