Protein aggregation in a mutant deficient in yajL, the bacterial homolog of the Parkinsonism-associated protein DJ-1.

The Journal of Biological Chemistry
Fatoum KthiriGilbert Richarme

Abstract

YajL is the closest prokaryotic homolog of the parkinsonism-associated protein DJ-1 (40% sequence identity and similar three-dimensional structure), a protein of unknown function involved in the cellular response to oxidative stress. We report here that a yajL mutant of Escherichia coli displays an increased sensitivity to oxidative stress. It also exhibits a protein aggregation phenotype in aerobiosis, but not in anaerobiosis or in aerobic cells overexpressing superoxide dismutase, suggesting that protein aggregation depends on the presence of reactive oxygen species produced by respiratory chains. The protein aggregation phenotype of the yajL mutant, which can be rescued by the wild-type yajL gene, but not by the corresponding cysteine 106 mutant allele, is similar to that of multiple mutants deficient in superoxide dismutases and catalases, although intracellular hydrogen peroxide levels were not increased in the yajL mutant, suggesting that protein aggregation in this strain does not result from a hydrogen peroxide detoxification defect. Aggregation-prone proteins included 17 ribosomal proteins, the ATP synthase beta subunit, flagellin, and the outer membrane proteins OmpA and PAL; all of them are part of multiprotein compl...Continue Reading

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Citations

Sep 4, 2010·Antioxidants & Redox Signaling·Mark A Wilson
Jan 8, 2013·Journal of Bacteriology·Nadia MessaoudiGilbert Richarme
Apr 25, 2014·BMC Evolutionary Biology·Qiaoqiao ZhaoYing Huang
Dec 20, 2015·Biochimica Et Biophysica Acta·Thorsten FriedrichSabrina Burschel
Jan 18, 2016·Biochemical and Biophysical Research Communications·Jad AbdallahGilbert Richarme
Dec 19, 2015·FEMS Microbiology Letters·Changhan LeeChankyu Park
Jun 24, 2015·Biochemical and Biophysical Research Communications·Mouadh MihoubGilbert Richarme
Aug 18, 2016·Biochemical and Biophysical Research Communications·Gilbert RicharmeYoshizumi Ishino
Jun 10, 2017·Science·Gilbert RicharmeAazdine Lamouri
Aug 6, 2017·The Journal of Biological Chemistry·Daniel H PfaffAurelio A Teleman
Mar 23, 2021·The Journal of Cell Biology·Nikhil PanickerTed M Dawson

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