Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli)

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Jee-Hyun JungChang-Hee Han

Abstract

To investigate the in vivo estrogenic activity of 4-nonylphenol (4-NP) on reproductive function, we have measured the expression level of rockfish vitellogenin (rVTG) mRNA, concentration of plasma VTG and levels of plasma estradiol-17beta(E(2)) and testosterone (T) by radioimmunoassay (RIA). An rVTG mRNA transcript of approximately 4.0 kb was extracted from hepatic tissue for Northern blot analysis, and the effects of 4-NP on rVTG mRNA expression in vivo with both male and female juvenile rockfish were examined. The level of rVTG mRNA expression was increased 48 h after injection with 4-NP of 10 mg/kg body mass and after injection in both male and female rockfish. The level of rVTG mRNA expression was increased 24 h after injection in male rockfish injected with 4-NP of 25 mg/kg body mass, while the level of rVTG mRNA expression was increased 12h after injection in female injected with same dosage. Concentrations of plasma rVTG in female injected with 4-NP of 10 and 25 mg/kg b.w. were increased 72 h after injection and reached 35 and 66 mg/mL, respectively. In male, rVTG concentrations with the dosage of 25 mg/kg body mass were increased 72 h after injection and reached to 14 and 65 mg/mL at 168 h, respectively. Plasma concentr...Continue Reading

References

Sep 1, 1988·General and Comparative Endocrinology·K Le GuellecM Tenniswood
Oct 1, 1995·Environmental Health Perspectives·J P Sumpter, S Jobling
Apr 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J J LechL Ren
Feb 28, 1998·Environmental Health Perspectives
Dec 21, 2000·General and Comparative Endocrinology·C J BowmanN D Denslow
Aug 21, 2003·Ecotoxicology and Environmental Safety·K Van den BeltH Witters
May 12, 2004·Reproductive Toxicology·Marco CardinaliOliana Carnevali
Jun 24, 2004·Chemosphere·Zhengyan LiJong-Geel Je
Nov 18, 2005·Marine Pollution Bulletin·Donghao LiSung Rok Cho

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Citations

Jun 13, 2012·International Journal of Environmental Research and Public Health·Eui-Man JungEui-Bae Jeung
Aug 27, 2014·Fish Physiology and Biochemistry·Wen Ting SongHong Cai Liu
Aug 23, 2011·General and Comparative Endocrinology·Valentina PomattoMaria Fosca Franzoni
Nov 26, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yuanxiang JinZhengwei Fu
May 5, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jee Hyun JungWon Joon Shim
Sep 7, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·B J KangC-H Han

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