Protein arginine methylation during lytic adenovirus infection

The Biochemical Journal
J KzhyshkowskaT Dobner

Abstract

Arginine methylation of proteins affects major processes in the cell, including transcriptional regulation, mRNA metabolism, signal transduction and protein sorting. Arginine methylation of Ad (adenovirus) E1B 55-kDa-associated protein E1B-AP5 was recently described by us [Kzhyshkowska, Schutt, Liss, Kremmer, Stauber, Wolf and Dobner (2001) Biochem. J. 358, 305-314]. In this first example of protein arginine methylation analysis in Ad-infected cells, we investigated methylation of the E1B-AP5 and the viral L4-100 kDa protein. We demonstrate that E1B-AP5 methylation is enhanced during the course of infection in a cell-type-specific manner. We also show that L4-100 kDa is efficiently methylated in Ad-infected cells. L4-100 kDa formed complex with methyltransferase in vivo during productive infection, and can be methylated by HRMT1L2 (human protein arginine methyltransferase 1) in vitro. Comparative analysis of E1B-AP5 and L4-100 kDa protein methylation in Ad-infected HeLa, MCF-7 and H1299 cells revealed that the profile of protein arginine methylation correlates with the efficiency of Ad proteins production. Our results suggest that protein arginine methylation is an important host-cell function required for efficient Ad replicat...Continue Reading

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Citations

Feb 17, 2005·Science's STKE : Signal Transduction Knowledge Environment·François-Michel BoisvertStéphane Richard
Aug 10, 2007·Journal of Virology·Demetris C IacovidesFrank McCormick
Dec 16, 2011·Journal of Virology·Melanie SchmidThomas Dobner
Dec 16, 2006·Retrovirology·Nicole M WillemsenDavid Harrich
May 26, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ayaz Ahmad, Xiaofeng Cao
Oct 4, 2005·Molecular and Biochemical Parasitology·Michel PelletierLaurie K Read
May 29, 2015·Scientific Reports·Gayathri GurunathanStéphane Richard
Nov 6, 2015·Journal of Virology·Carolin BürckSabrina Schreiner
May 9, 2008·Journal of Biochemistry·Robert B Denman

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