Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus

Genes & Development
Shi-Bin HuLing-Ling Chen

Abstract

In many cells, mRNAs containing inverted repeated Alu elements (IRAlus) in their 3' untranslated regions (UTRs) are inefficiently exported to the cytoplasm. Such nuclear retention correlates with paraspeckle-associated protein complexes containing p54(nrb). However, nuclear retention of mRNAs containing IRAlus is variable, and how regulation of retention and export is achieved is poorly understood. Here we show one mechanism of such regulation via the arginine methyltransferase CARM1 (coactivator-associated arginine methyltransferase 1). We demonstrate that disruption of CARM1 enhances the nuclear retention of mRNAs containing IRAlus. CARM1 regulates this nuclear retention pathway at two levels: CARM1 methylates the coiled-coil domain of p54(nrb), resulting in reduced binding of p54(nrb) to mRNAs containing IRAlus, and also acts as a transcription regulator to suppress NEAT1 transcription, leading to reduced paraspeckle formation. These actions of CARM1 work together synergistically to regulate the export of transcripts containing IRAlus from paraspeckles under certain cellular stresses, such as poly(I:C) treatment. This work demonstrates how a post-translational modification of an RNA-binding protein affects protein-RNA intera...Continue Reading

References

Jun 26, 1999·Science·D ChenM R Stallcup
Jan 16, 2002·Current Biology : CB·Archa H FoxAngus I Lamond
Sep 9, 2005·Molecular Biology of the Cell·Archa H FoxAngus I Lamond
Oct 22, 2005·Cell·Kannanganattu V PrasanthDavid L Spector
Jun 9, 2006·The Journal of General Virology·Sougata SahaPundi N Rangarajan
Jan 12, 2008·EMBO Reports·Neelu YadavMark T Bedford
May 24, 2008·The EMBO Journal·Ling-Ling ChenGordon G Carmichael
Jan 20, 2009·Molecular Cell·Mark T Bedford, Steven G Clarke
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yasnory T F SasakiTetsuro Hirose
Mar 11, 2009·Current Protocols in Bioinformatics·Maja Tarailo-Graovac, Nansheng Chen
Apr 3, 2009·Current Opinion in Genetics & Development·Rui ZhaoDavid L Spector
Sep 2, 2009·The Journal of Cell Biology·Charles S Bond, Archa H Fox
Dec 21, 2010·Nature Cell Biology·Yuntao S MaoDavid L Spector
Dec 22, 2010·Molecular Cell·Yanzhong YangMark T Bedford
Feb 9, 2011·Nucleic Acids Research·Hidekazu IiokaIan G Macara
Jun 18, 2011·Trends in Genetics : TIG·Yuntao S MaoDavid L Spector
Sep 1, 2012·Nature Protocols·Jianxing FengXiaole Shirley Liu
Sep 11, 2012·Molecular Cell·Qing-Fei YinLing-Ling Chen
Dec 19, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jia LiEllen R Richie
Jul 6, 2013·Cell·Igor Ulitsky, David P Bartel
Sep 17, 2013·Molecular Cell·Yang ZhangLing-Ling Chen
Nov 1, 2013·Molecular Biology of the Cell·Tetsuro HiroseGérard Pierron
Jul 25, 2014·PLoS Computational Biology·Rekin's JankyStein Aerts
Aug 27, 2014·Molecular Cell·Jason A WestRobert E Kingston
Nov 8, 2014·Nucleic Acids Research·Qing-Fei YinLing-Ling Chen

❮ Previous
Next ❯

Citations

Feb 26, 2016·Science·Reyad A ElbarbaryLynne E Maquat
Jul 8, 2015·Chromosoma·Mansoureh TajaddodKonstantin Licht
Apr 17, 2016·Nucleic Acids Research·Gavin J KnottArcha H Fox
Sep 21, 2016·The Journal of Cell Biology·Shi-Bin HuLing-Ling Chen
Sep 27, 2016·Scientific Reports·Aparna AnantharamanKannanganattu V Prasanth
Nov 22, 2016·Nature Reviews. Genetics·Edward B ChuongCédric Feschotte
Jan 4, 2017·Expert Review of Proteomics·Chao Peng, Catherine Cl Wong
Jan 7, 2017·Nucleus·Manon TorresAnne-Marie François-Bellan
Jan 25, 2017·Nucleic Acids Research·Guozhen GaoMark T Bedford
May 18, 2017·Nature Reviews. Molecular Cell Biology·Jernej Murn, Yang Shi
Mar 24, 2017·Nucleic Acids Research·John KarijolichBritt Glaunsinger
Nov 16, 2018·Molecular Oncology·Christiane KlecMartin Pichler
May 3, 2019·Nature Cell Biology·Run-Wen YaoLing-Ling Chen
Jul 4, 2019·Nature Reviews. Molecular Cell Biology·Ashwini JambhekarYang Shi
Mar 14, 2020·Journal of Cellular and Molecular Medicine·Peifu FengMao Ye
Oct 28, 2016·IUBMB Life·Yu-Hang XingLing-Ling Chen
Oct 6, 2015·Protein Science : a Publication of the Protein Society·Gavin J KnottCharles S Bond
Aug 25, 2020·Essays in Biochemistry·Yang Wang, Ling-Ling Chen
Jan 6, 2017·Nucleic Acids Research·Aparna AnantharamanKannanganattu V Prasanth
Nov 21, 2018·Life Science Alliance·Donghang ChengMark T Bedford
Jul 28, 2019·Nature Reviews. Molecular Cell Biology·Ernesto Guccione, Stéphane Richard
Mar 29, 2020·Cellular and Molecular Life Sciences : CMLS·Ziqiang WangWeiren Huang
Sep 4, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kentaro IshidaShinichi Nakagawa
Jan 25, 2021·Trends in Cell Biology·Samyuktha SureshThierry Dubois
Mar 9, 2021·RNA Biology·Chong Tong, Yafei Yin
Apr 2, 2021·Annual Review of Genomics and Human Genetics·Xiao-Ou ZhangZhiping Weng
Apr 4, 2015·Genes & Development·Reyad A Elbarbary, Lynne E Maquat

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.