Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent

Optics Letters
Changhuei YangJoseph A Izatt

Abstract

We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and 83 microM of phyA.

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Citations

Feb 18, 2014·Survey of Ophthalmology·J Emanuel Ramos de CarvalhoReinier O Schlingemann
Oct 27, 2005·The British Journal of Radiology·A Gh Podoleanu
Sep 13, 2006·Journal of Biomedical Optics·Anant AgrawalT Joshua Pfefer
Nov 13, 2007·Journal of Biomedical Optics·Adam M ZyskStephen A Boppart
May 10, 2008·Journal of Biomedical Optics·Matti KinnunenSeppo Vainio
Sep 24, 2005·Journal of Biomedical Optics·Stephen A BoppartDaniel L Marks
Dec 14, 2004·Photochemistry and Photobiology·Changhuei Yang
Nov 23, 2006·Nature Medicine·Seok H YunBrett E Bouma
Dec 13, 2006·Progress in Retinal and Eye Research·Mirjam E J van VelthovenMarc D de Smet
Oct 7, 2004·Current Opinion in Neurobiology·Amit D MehtaMark J Schnitzer
Oct 10, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Martina GiannacciniVittoria Raffa
Oct 17, 2014·Applied Optics·JaeHwang JungYongKeun Park
Apr 19, 2005·Optics Letters·Amy L OldenburgStephen A Boppart
May 9, 2015·Physics in Medicine and Biology·Jina KimAdam Wax
Jul 11, 2006·Applied Optics·Linbo Liu, Nan Guang Chen
Mar 26, 2005·Optics Letters·Jeremy S BredfeldtStephen A Boppart
Feb 15, 2017·Analytical Chemistry·Xueqin JiangJianhua Zhou
Feb 6, 2020·ACS Nano·George J LuMikhail G Shapiro

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