Protein C assay performance: an analysis of North American specialized coagulation laboratory association proficiency testing results

American Journal of Clinical Pathology
Jason M BaronElizabeth M Van Cott

Abstract

To determine the performance and frequency of protein C reagents currently used by clinical laboratories, we analyzed North American Specialized Coagulation Laboratory Association (NASCOLA) protein C proficiency testing data from 6 surveys conducted in 2009 and 2010 (2009-1 to 2009-3 and 2010-1 to 2010-3). Interlaboratory coefficients of variation (CV) for commonly used reagents on a survey with normal protein C ranged from 8% to 12% for antigenic assays, from 4% to 7% for chromogenic activity assays, and from 7% to 22% for clot-based activity assays. CVs for commonly used reagents on specimens with abnormal protein C ranged from 15% to 24% for antigenic, 4% to 11% for chromogenic, and 10% to 17% for clot-based assays (averaged across 3 surveys). Some reagents were used by relatively few laboratories and therefore additional study may be needed for those reagents. For all commonly used reagents, biases were usually small and often not statistically significant. All assessed reagents were clinically accurate, and were considered acceptable options for a specialized coagulation laboratory.

References

Jun 29, 2005·American Journal of Clinical Pathology·Elizabeth M Van CottUNKNOWN NASCOLA Proficiency Testing Committee
Aug 12, 2009·Clinics in Laboratory Medicine·Bernard Khor, Elizabeth M Van Cott
Mar 24, 2010·American Journal of Hematology·Bernard Khor, Elizabeth M Van Cott
Nov 26, 2010·American Journal of Hematology·Bernard Khor, Elizabeth M Van Cott
Mar 23, 2011·International Journal of Laboratory Hematology·P C CooperA D Mumford

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Citations

Jun 18, 2016·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Mariya NegrevaKaterina Vitlianova
Apr 19, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Emmanuel J FavaloroPiet Meijer
May 7, 2019·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Tariq M RoshanXiu Y Jiang
Mar 14, 2013·International Journal of Laboratory Hematology·C P M Hayward
Feb 1, 2019·Archives of Pathology & Laboratory Medicine·Peyman Dinarvand, Karen A Moser
Jul 28, 2020·Thrombosis and Haemostasis·Martine Alhenc-GelasUNKNOWN GFHT Study Group on Genetic Thrombophilia
May 16, 2015·International Journal of Laboratory Hematology·N D ZantekE M Van Cott
Jun 13, 2021·International Journal of Laboratory Hematology·Catherine P M Hayward, Kristi J Smock

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