Protein Carbamylation: A Marker Reflecting Increased Age-Related Cell Oxidation

International Journal of Molecular Sciences
J CarracedoRafael Ramírez-Chamond

Abstract

Carbamylation is a post-translational modification of proteins that may partake in the oxidative stress-associated cell damage, and its increment has been recently proposed as a "hallmark of aging". The molecular mechanisms associated with aging are related to an increased release of free radicals. We have studied whether carbamylated proteins from the peripheral blood of healthy subjects are related to oxidative damage and aging, taking into account the gender and the immune profile of the subjects. The study was performed in healthy human volunteers. The detection of protein carbamylation and malondialdehyde (MDA) levels was evaluated using commercial kits. The immune profile was calculated using parameters of immune cell function. The results show that the individuals from the elderly group (60⁻79 years old) have increased carbamylated protein and MDA levels. When considered by gender, only men between 60 and 79 years old showed significantly increased carbamylated proteins and MDA levels. When those subjects were classified by their immune profile, the carbamylated protein levels were higher in those with an older immune profile. In conclusion, the carbamylation of proteins in peripheral blood is related to age-associated o...Continue Reading

References

Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Jan 1, 1990·Methods in Enzymology·H Esterbauer, K H Cheeseman
Mar 1, 1990·Journal of Gerontology·S J WayneJ S Goodwin
Nov 1, 1989·Journal of the American Geriatrics Society·J K Cooper, C Gardner
Jul 27, 2000·Annals of the New York Academy of Sciences·B FriguetI Petropoulos
Jul 27, 2000·Annals of the New York Academy of Sciences·C FranceschiG De Benedictis
Aug 10, 2001·JAMA : the Journal of the American Medical Association·P Y LeeE D Peterson
Mar 19, 2002·BMC Geriatrics·Arnold B MitnitskiKenneth Rockwood
Aug 2, 2005·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Daniele Del RioNicoletta Pellegrini
Nov 23, 2006·Mechanisms of Ageing and Development·Sonya VastoCalogero Caruso
Apr 27, 2007·Annals of the New York Academy of Sciences·Syed Ibrahim Rizvi, Pawan Kumar Maurya
May 22, 2007·Journal of the American College of Cardiology·Tessa J MocattaChristine C Winterbourn
Sep 11, 2007·Nature Medicine·Zeneng WangStanley L Hazen
May 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael RistowMatthias Blüher
Sep 17, 2009·Current Pharmaceutical Design·Mónica De la Fuente, Jaime Miquel
Jan 1, 2010·Rejuvenation Research·Karen Bandeen-RocheLuigi Ferrucci
Jan 13, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Silvia GiovanniniFrancesco Landi
May 22, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Piotr MydelMaria Bokarewa
Aug 19, 2010·Oxidative Medicine and Cellular Longevity·Kanti Bhooshan Pandey, Syed Ibrahim Rizvi
Oct 23, 2010·Molecular Cell·Marcia C Haigis, Bruce A Yankner
Nov 19, 2010·The Journal of Nutrition, Health & Aging·K ZhuR L Prince
Jan 14, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julia CarracedoRafael Ramírez
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·James M RobertsJoost de Gouw
Oct 17, 2012·PloS One·Maciej S BuchowskiL Jackson Roberts
Dec 21, 2013·The Journal of Nutrition·Katherine Gray-DonaldHélène Payette
Mar 25, 2014·European Heart Journal·Thimoteus SpeerFelix C Tanner
May 17, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Claudio Franceschi, Judith Campisi
May 29, 2014·Cell Cycle·Xavier Deschênes-SimardGerardo Ferbeyre
Jun 19, 2014·Kidney International·Dalia El-GamalGunther Marsche
Jul 8, 2014·Oxidative Medicine and Cellular Longevity·Antonio AyalaSandro Argüelles
Mar 19, 2015·Maturitas·Sonia CabrerizoVincenzo Malafarina
Mar 31, 2015·Mechanisms of Ageing and Development·Alexander BürkleRichard Aspinall
Jun 11, 2015·Kidney International·Frederik H VerbruggeStanley L Hazen
Jun 14, 2015·Annals of Clinical Biochemistry·Gary WeavingRichard G Jones
Sep 2, 2015·Medycyna pracy·Marta CzerskaWojciech Wąsowicz
Nov 27, 2015·The Journal of Physiology·Guillermo López-Lluch, Plácido Navas
Dec 15, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Paola Lucia MinciulloGiorgio Basile
Dec 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Laëtitia GorissePhilippe Gillery
Jan 17, 2016·Mechanisms of Ageing and Development·Moisés Evandro Bauer, Mónica De la Fuente
Apr 30, 2016·Oxidative Medicine and Cellular Longevity·Bojana KisicLjiljana Popovic
Sep 3, 2016·The Journal of Biological Chemistry·Zeneng WangStanley L Hazen
Dec 3, 2016·Aging·Irene Martínez de TodaMónica De la Fuente

❮ Previous
Next ❯

Citations

Nov 30, 2019·International Journal of Molecular Sciences·Mónica De la Fuente
Apr 11, 2020·Biogerontology·Karolin YanarUfuk Çakatay
Sep 27, 2020·Cells·Gisela Machado-OliveiraOtília V Vieira
Jan 5, 2021·Frontiers in Cell and Developmental Biology·Francesco Demetrio LofaroDaniela Quaglino
Dec 10, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F A RwatambugaD D Tshala-Katumbay
Apr 29, 2021·Antioxidants & Redox Signaling·Maria Isabel Trejo-ZambranoFelipe Andrade

❮ Previous
Next ❯

Methods Mentioned

BETA
MDA
Assay
ELISA
protein assay

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.