Protein characterization using electrophoresis and immunofixation; a case-based review of dogs and cats

Veterinary Clinical Pathology
A Russell Moore, Paul R Avery


Protein electrophoresis and immunotyping can be a useful adjunct to the standard biochemical techniques for characterizing serum and urine proteins. This paper reviews currently available and commonly used methods for diagnostic protein electrophoresis, including both agarose gel and capillary zone electrophoretic techniques and total protein assessments. Immunofixation and immunosubtraction methods for identification of immunoglobulin location and class are also presented. Practical application of quality assurance and quality control strategies in compliance with American Society of Veterinary Clinical Pathology (ASVCP) best practices are discussed. Commonly encountered serum and urine electrophoretic diagnostic patterns, including electrophoretically normal, acute-phase protein responses, polyclonal gammopathies, restricted polyclonal/oligoclonal gammopathies, paraproteinemias (monoclonal or biclonal gammopathies), and Bence-Jones proteinurias are also reviewed using relevant case material. Cases in which immunofixation electrophoresis are particularly useful are highlighted, and methodologies to more accurately quantify serum monoclonal proteins (M-proteins), monitoring tests commonly used in human medicine, are discussed.


Dec 1, 1989·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·G L BrackeenC L Long
May 1, 1988·Journal of Comparative Pathology·G O Evans, C E Parsons
Jan 1, 1987·Journal of Veterinary Internal Medicine·E B BreitschwerdtO Barta
Aug 1, 1986·Nihon juigaku zasshi. The Japanese journal of veterinary science·T Furukawa, F Sugiyama
Mar 1, 1986·Veterinary Pathology·R M JacobsM L Wellman
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·C B Laurell
Aug 1, 1972·The British Veterinary Journal·G W Osbaldiston
Dec 1, 1981·The American Journal of Medicine·R A KyleJ A Katzmann
Oct 1, 1982·Equine Veterinary Journal·A G Matthews
May 1, 1994·Journal of Veterinary Internal Medicine·A FontJ Mascort
Sep 1, 1995·Journal of Veterinary Internal Medicine·M J BurkhardP C Schultheiss
Jan 1, 1997·Journal of the American Animal Hospital Association·E N Peterson, A C Meininger
Nov 15, 1996·Veterinary Parasitology·S HarrusH Bark
Jul 14, 1998·Veterinary Immunology and Immunopathology·A J GermanM J Day
Dec 5, 1998·Veterinary Immunology and Immunopathology·R HarleyM J Day
Mar 14, 2000·The Veterinary Journal·O AbateU Dotta
Mar 23, 2002·Journal of the American Animal Hospital Association·Jérĵme M GiraudelJean-François Guelfi
Jun 1, 2002·Veterinary Clinical Pathology·Shashi K RamaiahRose E Raskin
Jun 19, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Xavier BossuytPierre Wallemacq
Apr 22, 2004·Veterinary Research·Henrik Hagbard PetersenPeter Mikael Helweg Heegaard
Aug 31, 2004·Veterinary Clinical Pathology·Younès ChorfiArmand Tremblay
Jan 1, 1986·Veterinary Clinical Pathology·R J Baker, V E Valli
May 20, 2005·Veterinary Clinical Pathology·Jose Joaquýn CeronSilvia Martýnez-Subiela
Aug 23, 2005·Journal of Feline Medicine and Surgery·Fikry Hanna
Oct 7, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Enrique BergónElena Miravalles
Nov 5, 2005·Veterinary Clinical Pathology·Manena FayosMaxey L Wellman
Nov 11, 2005·The American Journal of Gastroenterology·Georgios KalambokisEpameinondas V Tsianos
May 10, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Michele MussapMario Plebani
Jul 21, 2006·Leukemia·B G M DurieUNKNOWN International Myeloma Working Group
Feb 22, 2007·Veterinary Clinical Pathology·Fernanda Gomes Velasque GamaCláudia Aparecida da Silva Nogueira
May 4, 2007·International Journal of Laboratory Hematology·Kymberley Carter, Mark Worwood
Jul 4, 2007·American Journal of Veterinary Research·Pedro J Sánchez-CordónJosé C Gómez-Villamandos
Sep 19, 2007·American Journal of Clinical Pathology·Panagiotis A KonstantinopoulosBruce A Beckwith
Jul 8, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Christof SchildJean-Marc Nuoffer
Dec 6, 2008·Veterinary Clinical Pathology·Carolyn CrayKristopher L Arheart
Jan 30, 2009·Journal of Veterinary Internal Medicine·G BenchekrounD Rosenberg
Jun 1, 2005·Veterinary and Comparative Oncology·F GentiliniP Famigli Bergamini

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