Protein charge determination

Current Protocols in Protein Science
Donald J Winzor

Abstract

The most popular current method of determining protein valence entails the calculation of net charge from amino acid sequence/composition. However, the inaccuracy of that approach was recognized long before the advent of the protein data banks and computer programs to facilitate its adoption. Capillary zone electrophoresis affords the simplest and most economical procedure for obtaining a reliable estimate of the net charge of a protein in the buffer system of interest. This unit explains the major pitfalls in the calculation of net charge from protein sequence data.

References

Nov 15, 1991·Analytical Chemistry·B J Compton, E A O'Grady
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Jun 23, 1995·Journal of Chromatography. a·F KálmánC Horváth
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J GaoG M Whitesides
Aug 1, 1960·Archives of Biochemistry and Biophysics·L B BARNETT, H B BULL
Sep 29, 2004·Analytical Biochemistry·Donald J WinzorStephen E Harding

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Citations

Dec 18, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chen WangScott D Boyd
Oct 8, 2020·International Journal of Biometeorology·Pablo Fdez-ArroyabeMichael Rycroft

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