Protein-chemical analysis of Bio-Oss bone substitute and evidence on its carbonate content

Biomaterials
D BenkeH Möhler

Abstract

The natural bone substitute Bio-Oss is used in periodontal and maxillofacial surgery to fill bone defects and permit reossification. Recent reports have suggested the presence of TGFbeta and of substantial amounts of protein in Bio-Oss and have questioned its position as a biologically inert material and its safety in clinical applications (Hönig et al., Plast Reconstr Surg 1999;103:1324; Schwartz et al., J Periodontol 2000;71:1258). Bio-Oss was therefore subjected to a detailed biochemical, histochemical and biophysical analysis. In three different types of extracts of Bio-Oss no evidence for the presence of protein based on SDS-PAGE and silver staining was detected. In addition, as shown by Western blotting, there was no immunochemical evidence for the presence of the potential growth-inducing factor TGFbeta. Furthermore, micropolished sections of Bio-Oss failed to be stained with McNeal's Tetrachrome as did microtome sections treated with Goldner's Trichrome. However, Bio-Oss was strongly stained with the protein dye Coomassie blue. This staining was virtually irreversible and is attributed to the carbonate content of Bio-Oss which was detected by thermogravimetry-mass spectrometry. Thus, within the limits of the assay condi...Continue Reading

References

Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T K SampathA H Reddi
Dec 1, 1985·Analytical Biochemistry·S J Compton, C G Jones
May 1, 1996·The Journal of the American Dental Association·G J Christensen
Mar 24, 1999·Plastic and Reconstructive Surgery·J F HönigD E Heinemann
Sep 6, 2000·Journal of Periodontology·Z SchwartzB D Boyan

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Citations

Jan 29, 2011·Calcified Tissue International·Po-Yu ChenJoanna McKittrick
Mar 12, 2010·Tissue Engineering. Part C, Methods·Emma L SmithAlastair J Sloan
Jun 24, 2004·Journal of Periodontology·S ErsanliB Leblebicioglu
Mar 16, 2004·Clinical Oral Implants Research·Michael R NortonRichard J Cook
Jul 8, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marie-Therese RameisReinhard Gruber
May 15, 2004·Clinical Oral Implants Research·Fabricio I TapetyTakeyasu Maeda
Feb 2, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·A DashtiS N Nazhat
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Ana B Castro-CeseñaJoanna McKittrick
Jan 5, 2010·Biomaterials·Faleh TamimiEnrique L Cabarcos
Feb 24, 2011·Journal of Biomaterials Applications·Etiene Andrade MunhozOsny Ferreira
Feb 19, 2020·The Journal of Oral Implantology·Sarah Al-MaawiShahram Ghanaati
Nov 1, 2013·The Journal of Contemporary Dental Practice·Danilo Barral AraujoRoberto Paulo Correia Araujo
Apr 25, 2015·The Journal of Contemporary Dental Practice·Danilo Alessio Di StefanoLaura Pieri

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