Protein corona mitigates the cytotoxicity of graphene oxide by reducing its physical interaction with cell membrane

Nanoscale
Guangxin DuanRuhong Zhou

Abstract

Many recent studies have shown that the way nanoparticles interact with cells and biological molecules can vary greatly in the serum-containing or serum-free culture medium. However, the underlying molecular mechanisms of how the so-called "protein corona" formed in serum medium affects nanoparticles' biological responses are still largely unresolved. Thus, it is critical to understand how absorbed proteins on the surfaces of nanoparticles alter their biological effects. In this work, we have demonstrated with both experimental and theoretical approaches that protein BSA coating can mitigate the cytotoxicity of graphene oxide (GO) by reducing its cell membrane penetration. Our cell viability and cellular uptake experiments showed that protein corona decreased cellular uptake of GO, thus significantly mitigating the potential cytotoxicity of GO. The electron microscopy images also confirmed that protein corona reduced the cellular morphological damage by limiting GO penetration into the cell membrane. Further molecular dynamics (MD) simulations validated the experimental results and revealed that the adsorbed BSA in effect weakened the interaction between the phospholipids and graphene surface due to a reduction of the available...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Sep 14, 2004·Science·Ruhong ZhouBruce J Berne
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
May 20, 2006·Science·Nathaniel L RosiChad A Mirkin
Oct 13, 2006·Journal of the American Chemical Society·Maria EleftheriouRuhong Zhou
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tommy CedervallSara Linse
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jingyuan LiRuhong Zhou
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Martin LundqvistKenneth A Dawson
Apr 16, 2009·The Journal of Physical Chemistry. B·Ruhong ZhouAjay K Royyuru
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Sep 8, 2009·Nature Nanotechnology·Iseult LynchKenneth A Dawson
Oct 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yusong TuHaiping Fang
May 26, 2010·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Jul 3, 2010·ACS Nano·Wenbing HuChunhai Fan
Oct 12, 2010·ACS Nano·Omid Akhavan, Elham Ghaderi
Mar 10, 2011·The Journal of Physical Chemistry. B·Peng XiuRuhong Zhou
Mar 10, 2011·Nanomedicine·Liangzhu Feng, Zhuang Liu
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Payel DasRuhong Zhou
Oct 1, 2011·Chemical Research in Toxicology·Vanesa C SanchezAgnes B Kane
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Cuicui GeChunying Chen
Nov 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Chih-Jen ShihDaniel Blankschtein
Dec 24, 2011·Journal of the American Chemical Society·Carl D WalkeyWarren C W Chan
Sep 21, 2012·Acta Crystallographica. Section D, Biological Crystallography·Anna Bujacz
Oct 13, 2012·Chemical Society Reviews·Kai YangZhuang Liu
Jan 31, 2013·Journal of the American Chemical Society·Seung-gu KangRuhong Zhou
Apr 13, 2013·Angewandte Chemie·Alberto Bianco
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bin DaiRuhong Zhou
Jul 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yinfeng LiHuajian Gao
Sep 24, 2013·Nature Nanotechnology·Stefan TenzerRoland H Stauber
Jan 16, 2014·Chemical Research in Toxicology·Amedea B SeabraNelson Durán
Dec 19, 2014·Chemical Society Reviews·Yu ChenLianzhou Wang
Feb 1, 2015·Archives of Toxicology·Cuicui GeZhifang Chai

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Citations

Dec 29, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Kunal BhattacharyaBengt Fadeel
Jun 3, 2016·Microscopy Research and Technique·Xiuyuan HuYi Zhang
Jul 28, 2016·Biosensors & Bioelectronics·Huan ZhangXianqiang Mi
Aug 3, 2016·Biomaterials Science·Erhan KelesYuehe Lin
Sep 7, 2016·Journal of Applied Toxicology : JAT·Waqar MajeedAlexandru S Biris
Nov 2, 2016·Particle and Fibre Toxicology·Lingling OuLongquan Shao
Dec 8, 2016·Nanoscale·Qing Shao, Carol K Hall
Jan 13, 2017·Scientific Reports·Marco PelinMaurizio Prato
Apr 4, 2017·Journal of Biomedical Materials Research. Part a·Mojgan NejabatMohammad Ramezani
Feb 22, 2017·Scientific Reports·Guangxin DuanRuhong Zhou
Jun 15, 2018·Advanced Materials·Zhaoxu TuRainer Haag
Nov 30, 2018·Physical Chemistry Chemical Physics : PCCP·Zonglin GuRuhong Zhou
Apr 3, 2020·Archives of Toxicology·Feng XiaoliShao Longquan
May 19, 2020·PloS One·Ingrid SafinaAlexandru S Biris
Jun 25, 2020·Nanoscale·Nurul 'Ain AzmanJames Chen Yong Kah
Oct 6, 2019·Nature Communications·Xiang LuYu-Qiang Ma
Jul 1, 2017·Frontiers in Immunology·Sourav P MukherjeeBengt Fadeel
Aug 6, 2019·Frontiers in Systems Neuroscience·Kristina E Kitko, Qi Zhang
Dec 15, 2019·Materials·Justyna SekretarskaBarbara Strojny
Nov 21, 2018·Nanomaterials·Krzysztof TadyszakJagoda Litowczenko
Jan 1, 2018·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Hong-Ming Ding, Yu-Qiang Ma
Jan 1, 2019·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Anisha AnandChih-Ching Huang
Jun 27, 2020·Frontiers in Chemistry·Xin TianCuicui Ge
Sep 3, 2020·Royal Society Open Science·Md Toasin Hossain AunkorAbul Kalam Azad
Apr 8, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·V PalmieriM Papi
Apr 27, 2018·Interface Focus·Denver P LinklaterElena P Ivanova
Feb 23, 2019·Soft Matter·Siva DasettySapna Sarupria
Oct 3, 2020·Biotechnology and Applied Biochemistry·Elisa Fasoli
Dec 10, 2020·IScience·Tian Zhang, Pier-Luc Tremblay

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