Protein Discrimination using a Fluorescence Based Sensor Array of Thiacarbocyanine-GUMBOS

ACS Sensors
Rocío L PérezIsiah M Warner

Abstract

Sensitive and selective detection of proteins from complex samples has gained substantial interest within the scientific community. Early and precise detection of key proteins play an important role in potential clinical diagnosis, treatment of different diseases, as well as in proteomic research. In the study reported here, six different compounds belonging to a group of uniform materials based on organic salts (GUMBOS) have been synthesized using three thiacarbocyanine (TC) dyes and employed as fluorescent sensors. Fluorescent properties of micro- and nano- aggregates of these TC-based GUMBOS formed in phosphate buffer solutions are studied in the absence and presence of seven proteins. Fluorescence response patterns of these TC-based GUMBOS were analyzed using linear discriminant analysis (LDA). The constructed LDA model allowed discrimination of these seven proteins at various concentrations with 100% accuracy. The sensing and discrimination abilities of these TC-based GUMBOS were further evaluated in mixtures of two major proteins, i.e. human serum albumin (HSA) and hemoglobin (Hb). Fluorescent response patterns of these mixtures were analyzed using LDA. This model allowed discrimination of various mixtures with 100% of ac...Continue Reading

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Citations

Apr 23, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Kunpeng YinJuanjuan Peng
Sep 7, 2021·Chemical Communications : Chem Comm·Hongxu LiuS Thayumanavan

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
PCA

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