Protein disulfide isomerase A3-specific Th1 effector cells infiltrate colon cancer tissue of patients with circulating anti-protein disulfide isomerase A3 autoantibodies

Translational Research : the Journal of Laboratory and Clinical Medicine
Cristiana CaorsiFrancesco Novelli

Abstract

To investigate novel colorectal cancer (CRC)-associated antigens that could be targets of humoral or cellular responses, we analyzed the reactivity of serum from a long-surviving CRC patient (for more than 100 months of follow-up) in clinical remission, by serologic proteome analysis. Two-dimensional Western blotting (2D-WB) and mass spectrometry analysis revealed a strong reactivity of this serum against protein disulfide isomerase A3 (PDIA3). Anti-PDIA3 antibodies are not a diagnostic marker of CRC, 2D-WB and Luminex analysis revealed that they were equally present in about 10% of sera from healthy subjects and CRC patients. Kaplan-Meier analysis of survival in CRC patient cohort, after 48 months of follow-up, showed a trend of higher survival in patients with increased levels of autoantibodies to PDIA3. Therefore, the interplay between the presence of these antibodies and T-cell response was investigated. Peripheral blood T cells from CRC patients with high immunoglobulin G (IgG) reactivity to PDIA3 also secreted interferon gamma (IFN-γ) when stimulated in vitro with recombinant PDIA3, whereas those from CRC with low IgG reactivity to PDIA3 did not. PDIA3-pulsed dendritic cells efficiently induced proliferation and IFN-γ pro...Continue Reading

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Citations

Jul 1, 2016·Blood·Robert Flaumenhaft, Bruce Furie
Aug 17, 2019·Frontiers in Immunology·Eda K HollSmita K Nair
Aug 31, 2019·Biochemical and Biophysical Research Communications·Wu-Tao WangChao-Hui Sun
Mar 6, 2021·Frontiers in Immunology·Hao ZhangQuan Cheng
Oct 16, 2020·International Journal of Molecular Sciences·Safiah Ibrahim AlthurwiFazlul Huq

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