Protein disulphide isomerase is required for signal peptide peptidase-mediated protein degradation.

The EMBO Journal
Seong-Ok LeeKwangseog Ahn

Abstract

The human cytomegalovirus glycoprotein US2 induces dislocation of MHC class I heavy chains from the endoplasmic reticulum (ER) into the cytosol and targets them for proteasomal degradation. Signal peptide peptidase (SPP) has been shown to be integral for US2-induced dislocation of MHC class I heavy chains although its mechanism of action remains poorly understood. Here, we show that knockdown of protein disulphide isomerase (PDI) by RNA-mediated interference inhibited the degradation of MHC class I molecules catalysed by US2 but not by its functional homolog US11. Overexpression of the substrate-binding mutant of PDI, but not the catalytically inactive mutant, dominant-negatively inhibited US2-mediated dislocation of MHC class I molecules by preventing their release from US2. Furthermore, PDI associated with SPP independently of US2 and knockdown of PDI inhibited SPP-mediated degradation of CD3delta but not Derlin-1-dependent degradation of CFTR DeltaF508. Together, our data suggest that PDI is a component of the SPP-mediated ER-associated degradation machinery.

References

Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·B E GewurzD C Wiley
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·S FangA M Weissman
Jan 5, 2002·Molecular and Cellular Biology·Efrat RabinovichShoshana Bar-Nun
Jan 16, 2002·The Journal of Biological Chemistry·Benjamin E GewurzDomenico Tortorella
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Boyoun ParkKwangseog Ahn
May 8, 2002·Nature Reviews. Molecular Cell Biology·Billy TsaiTom A Rapoport
Jun 22, 2002·Science·Andreas WeihofenBruno Martoglio
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Lars Ellgaard, Ari Helenius
Aug 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ardythe A McCracken, Jeffrey L Brodsky
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martine T BarelEmmanuel J H J Wiertz
Jun 25, 2004·Nature·Brendan N Lilley, Hidde L Ploegh
Aug 17, 2004·The Journal of Biological Chemistry·Klara K ErikssonMaurizio Molinari
Sep 18, 2004·The Biochemical Journal·Samuel G CrawshawStephen High
Aug 16, 2005·Molecular Immunology·Martine T BarelEmmanuel J H J Wiertz
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brendan N Lilley, Hidde L Ploegh
Jun 21, 2006·The Journal of Cell Biology·Michele L ForsterBilly Tsai
Aug 1, 2006·The Journal of Biological Chemistry·Gerco C HassinkEmmanuel J Wiertz
Feb 10, 2007·The FEBS Journal·Hiderou Yoshida
Mar 4, 2008·Nature Cell Biology·Songyu Wang, Davis T W Ng

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Citations

Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michael B HarbutDoron C Greenbaum
Aug 13, 2010·The Journal of Biological Chemistry·Nia Soetandyo, Yihong Ye
Dec 7, 2011·Antioxidants & Redox Signaling·Adam M Benham
Aug 31, 2011·Cold Spring Harbor Perspectives in Biology·Kazutaka Araki, Kazuhiro Nagata
Dec 17, 2010·Journal of Virology·Christopher P Walczak, Billy Tsai
May 24, 2011·Neurology Research International·Adam K Walker, Julie D Atkin
Jun 24, 2010·Current Opinion in Cell Biology·Ramanujan S Hegde, Hidde L Ploegh
Mar 30, 2010·Seminars in Cell & Developmental Biology·Pekka MäättänenDavid Y Thomas
Dec 20, 2011·The FEBS Journal·Franziska KriegenburgRasmus Hartmann-Petersen
Sep 28, 2015·Trends in Cell Biology·Dönem Avci, Marius K Lemberg
Aug 9, 2015·Molecular Cell·Daisuke Morito, Kazuhiro Nagata
Jun 9, 2015·Frontiers in Cell and Developmental Biology·Sonam Parakh, Julie D Atkin
Jun 15, 2013·Cell Calcium·Tino PrellJulian Grosskreutz
Oct 9, 2013·Biochimica Et Biophysica Acta·Matthias VossRegina Fluhrer
Feb 16, 2017·Scientific Reports·Ji Yang KimJi-Eun Kim
Jul 28, 2019·International Journal of Molecular Sciences·Trishna ManandharChristina Bade-Doeding
May 26, 2017·Frontiers in Molecular Neuroscience·Hamideh ShahheydariJulie D Atkin
Jun 13, 2020·Cellular and Molecular Neurobiology·Shusheng Wu, Longfei Du
Nov 18, 2011·The Journal of Biological Chemistry·Chao WangChih-chen Wang
Oct 14, 2021·Microbiology and Molecular Biology Reviews : MMBR·Evelyne KohliCarmen Garrido

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