PMID: 8955284Dec 1, 1996Paper

Protein-DNA interactions in the ori region of the Mycobacterium fortuitum plasmid pAL5000

Journal of Bacteriology
P Stolt, N G Stoker

Abstract

Plasmid pAL5000 from Mycobacterium fortuitum encodes two proteins necessary for replication: RepA (307 amino acid residues) and RepB (119 residues). A single RNA species encoding these proteins was characterized, and its 5' end was defined. The proteins were expressed as maltose-binding protein fusions in Escherichia coli. The RepB protein was shown in vitro to bind specifically to a previously defined 435-bp region of pAL5000 containing the origin of replication (ori). The precise RepB binding sites were defined by DNase I footprinting experiments. RepB binds to two motifs in the ori region: a high-affinity site within its own promoter region, implying autoregulation of its expression, and a low-affinity site further upstream, presumably the origin of replication itself. The binding to the latter motif seems to occur on one DNA strand only. The high-affinity binding site contains several palindromic sequences. Gel retardation assays were performed with the different binding sites as templates, and the binding constant to each site was estimated from protein titrations. This is the first molecular dissection of mycobacterial DNA-binding proteins and their interactions with their targets.

References

Sep 23, 1976·Molecular & General Genetics : MGG·W Tacon, D Sherratt
Oct 25, 1992·Nucleic Acids Research·J HainW Zillig
Jun 6, 1991·Nature·C K StoverG F Hatfull
Jan 1, 1991·Methods in Enzymology·D HanahanF R Bloom
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B L Kittell, D R Helinski
Jan 1, 1989·Journal of Bacteriology·A L AbelesS J Austin
Jan 1, 1989·Molecular & General Genetics : MGG·H YasuedaT Itoh
Jan 1, 1989·Annual Review of Microbiology·R P Novick
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S B SnapperW R Jacobs
Sep 1, 1985·Annales De L'Institut Pasteur. Microbiology·A LabidiD Roulland-Dussoix
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D K ChattorajA L Abeles
Dec 24, 1970·Biochemical and Biophysical Research Communications·D T Kingsbury, D R Helinski
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D ChattorajA Abeles
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J W LittleC R Yanisch-Perron
Oct 25, 1995·Nucleic Acids Research·S Takechi, T Itoh
Jan 1, 1993·Bioscience, Biotechnology, and Biochemistry·T ArayaH Takahashi
Sep 1, 1994·Journal of Bacteriology·Y ZhangA J Pittard
Dec 1, 1994·Journal of Bacteriology·S HiragaT Itoh
Sep 1, 1995·Journal of Bacteriology·M L BeggsK D Eisenach
Jan 1, 1994·Journal of Bacteriology·M QinY Mizuguchi
Sep 1, 1995·Molecular Microbiology·L KremerC Locht

❮ Previous
Next ❯

Citations

Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael W PanasWilliam R Jacobs
Oct 10, 1997·Nucleic Acids Research·P Stolt, N G Stoker
Jan 7, 2003·Applied and Environmental Microbiology·Carey A PashleyNeil G Stoker
Oct 2, 2007·Journal of Bacteriology·Sujoy ChatterjeeSujoy K Das Gupta
May 9, 1998·Journal of Bacteriology·M D Bashyam, A K Tyagi
Jun 30, 2000·Plasmid·T AparicioJ Perera
Apr 1, 2014·Microbiology Spectrum·William R Jacobs

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.